Microscale plasma-initiated patterning of electrospun polymer scaffolds.
Microscale plasma-initiated patterning of electrospun polymer scaffolds.
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电纺聚合物支架的微尺度等离子体引发图案化。
DOI：
10.1016/j.colsurfb.2011.01.014
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Uhrich,KathrynE
中科院分区：
文献类型：
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作者：
Delgado-Rivera,Roberto;Griffin,Jeremy;Ricupero,ChristopherL;Grumet,Martin;Meiners,Sally;Uhrich,KathrynE
微尺度等离子体引发图案化（μPIP）是一种用于在聚合物表面形成生物分子微图案的新型微图案化技术。图案化方法使用聚二甲基硅氧烷（PDMS）印模来选择性地保护下面的衬底的区域免受氧等离子体处理，从而产生疏水和亲水区域。生物分子优先吸附到等离子体暴露（亲水）或等离子体保护（疏水）区域上导致生物分子微图案。在目前的工作中，层粘连蛋白-1施加到静电纺丝聚酰胺纳米纤维基质等离子体处理后。放射状胶质细胞克隆（神经前体）选择性地粘附到这些图案化的基质表面上的蛋白质的轮廓。这项工作表明，纹理表面，如纳米纤维支架，可以被微图案化，以提供细胞组织的外部化学线索。
Microscale plasma-initiated patterning (μPIP) is a novel micropatterning technique used to create biomolecular micropatterns on polymer surfaces. The patterning method uses a polydimethylsiloxane (PDMS) stamp to selectively protect regions of an underlying substrate from oxygen plasma treatment resulting in hydrophobic and hydrophilic regions. Preferential adsorption of the biomolecules onto either the plasma-exposed (hydrophilic) or plasma-protected (hydrophobic) regions leads to the biomolecular micropatterns. In the current work, laminin-1 was applied to an electrospun polyamide nanofibrillar matrix following plasma treatment. Radial glial clones (neural precursors) selectively adhered to these patterned matrices following the contours of proteins on the surface. This work demonstrates that textured surfaces, such as nanofibrillar scaffolds, can be micropatterned to provide external chemical cues for cellular organization.