Measurement of free testosterone in normal women and women with androgen deficiency: Comparison of methods
Measurement of free testosterone in normal women and women with androgen deficiency: Comparison of methods
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DOI：
10.1210/jc.2003-030680
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发表时间：
2004-02-01
影响因子：
5.8
通讯作者：
Klibanski, A
中科院分区：
文献类型：
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作者：
Miller, KK;Rosner, W;Klibanski, A
女性雄激素缺乏越来越被认为是一种新的临床综合征，并提高了我们对准确和有效测量女性血清游离睾酮(T)浓度的重要性的认识。因此，我们将平衡透析测量的血清游离T水平与直接RIA（模拟方法）测量的血清游离T水平以及根据质量作用定律（需要测量总T和SHBG）计算的血清游离T水平进行了比较。我们还计算了游离雄激素指数，100 × T/SHBG，作为一个已知的与游离T相关的简单指数。研究对象是147名雄激素和雌激素状态不同的女性。所有患者在1个月内进行了3次研究，包括1)月经规律（雌激素阳性，T阳性），2)50岁以上未接受雌激素治疗（雌激素阴性，T阳性），3)接受雌激素治疗（雌激素阳性，T阴性），4)垂体功能低下继发严重雄激素缺乏（雌激素阴性，T阴性）的妇女。利用质量作用定律计算出的游离T值与平衡透析得到的值有很好的相关性(r=0.99； P
Androgen deficiency in women is increasingly recognized as a new clinical syndrome and has raised our awareness of the importance of accurate and well-validated measurements of serum free testosterone (T) concentrations in women. Therefore, we compared serum free T levels measured by equilibrium dialysis to those measured by a direct RIA (analog method) and to those calculated from the law of mass action (requires the measurement of total T and SHBG). We also calculated the free androgen index, 100 x T/SHBG, as a simple index known to correlate with free T. Subjects were 147 women with variable androgen and estrogen statuses. All were studied three times in 1 month and included women 1) with regular menses (estrogen positive, T positive), 2) more than 50 yr old and not receiving estrogen (estrogen negative, T positive), 3) receiving estrogen (estrogen positive, T negative), and 4) with severe androgen deficiency secondary to hypopituitarism (estrogen negative, T negative). Calculated values for free T using the laws of mass action correlated well with those obtained from equilibrium dialysis (r=0.99; P