Changes in cholecystokinin mRNA expression in methamphetamine-induced behavioral sensitization
Changes in cholecystokinin mRNA expression in methamphetamine-induced behavioral sensitization
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DOI：
10.1016/0197-0186(95)00104-2
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发表时间：
1996-04-01
影响因子：
4.2
通讯作者：
Fukamauchi, F
中科院分区：
文献类型：
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作者：
Fukamauchi, F
在亚慢性给予甲基苯丙胺(MAP)的大鼠脑内，测定了神经肽CCK(CCK)mRNA的表达。雄性Wistar大鼠每日注射MAP(3 mg/kg或6 mg/kg，每天1次)或生理盐水，连续14天。在MAP诱导的刻板行为中观察到渐进性强化。停药7天或14天后，断头取脑，用P-32标记的cDNA探针进行Northern印迹分析。Northern印迹分析显示，与生理盐水对照组相比，3 mg/kg和6 mg/kg MAP组大鼠额叶皮质和海马区CCK mRNA的表达水平显著降低。这些结果表明，MAP大鼠额叶皮质和海马区CCK mRNA水平的变化持续至少2周，可能参与了MAP诱导的长时间行为敏化(反向耐受)的表达。(C)1996年爱思唯尔科学有限公司。
Neuropeptide cholecystokinin (CCK) mRNA was measured in the rat brain subchronically treated with methamphetamine (MAP). Male Wistar rats were injected daily with MAP (3 or 6 mg/kg, i.p., once a day) or saline for 14 days. Progressive reinforcement was observed in MAP-induced stereotyped behaviors. After 7 or 14 days of discontinuation, the rats were decapitated and the brains were prepared for Northern blot analysis using P-32-labeled cDNA probes. Northern blot analysis revealed that the levels of CCK mRNA in the frontal cortex and the hippocampus of 3 or 6 mg/kg MAP-treated rats were significantly decreased, compared to the saline-treated controls. These findings indicate that the alteration in CCK mRNA levels in the frontal cortex and the hippocampus of MAP-treated rats persisted for at least 2 weeks and might be involved in the expression of MAP-induced long-lasting behavioral sensitization (reverse tolerance). (C) 1996 Elsevier Science Ltd.