Decoding system for the AUA codon by tRNAIle with the UAU anticodon in Mycoplasma mobile.
Decoding system for the AUA codon by tRNAIle with the UAU anticodon in Mycoplasma mobile.
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DOI：
10.1093/nar/gks1344
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发表时间：
2013-02-01
影响因子：
14.9
通讯作者：
Suzuki T
中科院分区：
文献类型：
--
作者：
Taniguchi T;Miyauchi K;Nakane D;Miyata M;Muto A;Nishimura S;Suzuki T
破译遗传密码是所有生物体的基本过程。在许多细菌中，AUA密码子由在摆动位置处携带赖氨酸（L）的tRNAIle 2破译。L是由tRNAIle-赖氨酸合成酶（TilS）在转录后引入的修饰的胞苷。一些细菌，包括移动的支原体，不携带tilS基因，表明它们已经建立了不同的系统来解码AUA密码子。本研究从M.移动的，并发现含有UAU反密码子没有任何修饰。支原体移动的异亮氨酰-tRNA合成酶（IleRS）识别UAU反密码子，而大肠杆菌IleRS不能有效地氨酰化tRNAIle 2UAU。In M.在移动的IleRS中，第865位的一个Arg残基对UAU反密码子的特异性至关重要，当E. coliIleRS被Arg取代后，W 905 R突变体能有效地将tRNA氨酰化，并带有UAU反密码子。支原体移动的tRNAIle 2不能区分E.大肠杆菌核糖体。然而，在M.移动的核糖体，M.移动的tRNAIle 2UAU特异性识别AUA密码子，而不识别AUG密码子，提示M.移动的核糖体具有防止AUG密码子误读的特性。这些发现提供了一个深入了解的AUA解码系统的进化重组。
Deciphering the genetic code is a fundamental process in all living organisms. In many bacteria, AUA codons are deciphered by tRNAIle2 bearing lysidine (L) at the wobble position. L is a modified cytidine introduced post-transcriptionally by tRNAIle-lysidine synthetase (TilS). Some bacteria, including Mycoplasma mobile, do not carry the tilS gene, indicating that they have established a different system to decode AUA codons. In this study, tRNAIle2 has been isolated from M. mobile and was found to contain a UAU anticodon without any modification. Mycoplasma mobile isoleucyl-tRNA synthetase (IleRS) recognized the UAU anticodon, whereas Escherichia coli IleRS did not efficiently aminoacylate tRNAIle2UAU. In M. mobile IleRS, a single Arg residue at position 865 was critical for specificity for the UAU anticodon and, when the corresponding site (W905) in E. coli IleRS was substituted with Arg, the W905R mutant efficiently aminoacylated tRNA with UAU anticodon. Mycoplasma mobile tRNAIle2 cannot distinguish between AUA and AUG codon on E. coli ribosome. However, on M. mobile ribosome, M. mobile tRNAIle2UAU specifically recognized AUA codon, and not AUG codon, suggesting M. mobile ribosome has a property that prevents misreading of AUG codon. These findings provide an insight into the evolutionary reorganization of the AUA decoding system.
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DOI：
10.1073/pnas.0801980105
发表时间：
2008-06-10
影响因子：
11.1
作者：
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发表时间：
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影响因子：
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RajBhandary, Uttam L.
DOI：
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发表时间：
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