Expression of a novel dual-functional polypeptide and its pharmacological action research.
Expression of a novel dual-functional polypeptide and its pharmacological action research.
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DOI：
10.1016/j.lfs.2020.118890
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发表时间：
2020-12
期刊：
影响因子：
6.1
通讯作者：
Xiaodan Li;Xingkai Yang;Muhammad Umar;Zhixuan Zhang;W. Luo;Yu Fan;D. Ma;Minggang Li
中科院分区：
文献类型：
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作者：
Xiaodan Li;Xingkai Yang;Muhammad Umar;Zhixuan Zhang;W. Luo;Yu Fan;D. Ma;Minggang Li
目的开发一种具有降血糖和抗血栓双重功能的药物。主要方法采用SOE-PCR法克隆长效胰升糖素样肽-1(5×GLP-1)和纳豆激酶(NK)，转化大肠杆菌获得GLP-1和NK融合多肽。利用小鼠模型研究融合多肽的降血糖和抗血栓活性。给BALB/C小鼠腹腔注射角叉菜胶诱导尾部血栓模型。采用2型糖尿病小鼠模型，研究融合多肽的降血糖作用。结果表明，融合多肽口服后能显著抑制血栓形成。连续给药15天后，试验组的空腹血糖降至接近正常水平。意义本研究对RolGLP-1和NK融合多肽的表达、纯化和功能活性进行了研究，为进一步研究其详细的药理作用机制和药物开发奠定了基础。
AimsTo develop a dual-functional medicine for hypoglycemic and anti-thrombus.Main methodsThe long-acting glucagon like peptide-1 (5×GLP-1) and nattokinase (NK) were cloned by SOE PCR and gained the GLP-1 and NK fusion polypeptide after transformed intoE.coli. Use of mice models for the hypoglycemic and anti-thrombus activity of the fusion polypeptide. Balb/C mice were given the carrageenan by intraperitoneal injection to induce tail thrombus models. Type 2 diabetes mellitus mice model was used to research the hypoglycemic function of the fusion polypeptide.Key findingsResults showed that the fusion polypeptide could significantly prevent thrombus formation after oral administration. Continuous administration for 15 days, fasting blood glucose levels of the experimental group decreased to nearly normal levels.SignificanceThe present study investigated the expression, purification and functional activity of the rolGLP-1 and NK fusion polypeptide, which provided a foundation for further studying the detailed pharmaceutical mechanism and drug development.