Bio-Inspired Preparation of Fibrin-Boned Bionanocomposites of Biomacromolecules and Nanomaterials for Biosensing
Bio-Inspired Preparation of Fibrin-Boned Bionanocomposites of Biomacromolecules and Nanomaterials for Biosensing
复制标题
用于生物传感的生物大分子和纳米材料的纤维蛋白骨生物纳米复合材料的仿生制备
DOI：
10.1002/adfm.201400458
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发表时间：
2014-08-20
影响因子：
19
通讯作者：
Yao, Shouzhuo
中科院分区：
文献类型：
--
作者：
Han, Fangfang;Qi, Xin;Yao, Shouzhuo
向自然学习是开发先进功能材料最有前途的方法之一。在这里，受血液凝固的启发，新型的纤维蛋白结合的生物纳米复合材料被报道为生物大分子和生物传感纳米材料的有效固定化基质。采用葡萄糖氧化酶(Gox)、金纳米粒子(AuNPs)和Fe3O4磁性纳米粒子(MNPs)作为生物大分子和纳米材料的模型。通过将凝血酶触发的纤维蛋白凝固与先进的表面修饰技术相结合，开发并评价了四种固定化策略。利用数字成像、紫外可见光谱、扫描/透射电子显微镜、电化学方法和N-2吸附-脱附等温线研究了各种生物纳米复合材料的形成、固定化效率和性能。纤维蛋白结合网络具有良好的生物相容性、良好的吸附性能、孔隙率和功能化能力，使生物纳米复合材料对AuNPs、MNPs和Gox的捕获率高(在给定条件下分别为99%、98%和57%)，并具有显著的传质和生物催化效率。因此，纤维蛋白结合的生物纳米复合材料在生物传感方面显示出巨大的潜力，例如纤维蛋白-AuNPs-GOx-戊二醛生物纳米复合材料修饰的金电极对葡萄糖(145mA cm(-2)mm(-1))具有高度的敏感性，允许检测下限低至25 nm，远远优于已报道的类似物。所提出的实验平台/策略可广泛应用于其他生物/纳米材料/器件的开发。
Learning from nature is one of the most promising ways to develop advanced functional materials. Here, inspired by blood coagulation, novel fibrin-boned bionanocomposites are reported as efficient immobilization matrices of biomacromolecules and nanomaterials for biosensing. Glucose oxidase (GOx), Au nanoparticles (AuNPs), and Fe3O4 magnetic nanoparticles (MNPs) are adopted as the model biomacromolecules and nanomaterials. By integrating the thrombin-triggered coagulation of fibrin with advanced surficial modification techniques, four kinds of immobilization strategies are developed and evaluated. Digital imaging, UV-vis spectroscopy, scanning/transmission electron microscopy, electrochemical methods, and N-2 adsorption-desorption isotherms are used to investigate the formation, immobilization efficiency, and performance of various bionanocomposites. The fibrin-boned networks show inherent biocompatibility, excellent adsorbability, porosity, and functionalization ability, endowing the bionanocomposites with high efficiencies in capturing AuNPs, MNPs and GOx (99%, 98%, and 57% captured under the given conditions, respectively), as well as significant mass-transfer and biocatalysis efficiencies. Therefore, the fibrin-boned bionanocomposites show great potential for biosensing, for example, a fibrin-AuNPs-GOx-glutaraldehyde bionanocomposites modified Au electrode is highly sensitive to glucose (145 mu A cm(-2) mM(-1)) allowing for a limit of detection down to 25 nM, being much superior to those of the reported analogues. The presented experimental platform/strategy may find wide applications in the development of other bio/nano-materials/devices.