Independence of Genetic Geographical Variation between Photoperiodic Diapause, Circadian Eclosion Rhythm, and Thr-Gly Repeat Region of the Period Gene in Drosophila littoralis
Independence of Genetic Geographical Variation between Photoperiodic Diapause, Circadian Eclosion Rhythm, and Thr-Gly Repeat Region of the Period Gene in Drosophila littoralis
复制标题
果蝇光周期滞育、昼夜羽化节律和周期基因Thr-Gly重复区之间遗传地理变异的独立性
DOI：
--
复制
发表时间：
2006
影响因子：
3.5
通讯作者：
P. Forsman
中科院分区：
文献类型：
--
作者：
P. Lankinen;P. Forsman
小果蝇是一种分布在欧洲纬度地区的果蝇属物种。该种在光周期(成虫滞育)和昼夜节律(蛹羽化节律)方面有丰富的遗传变异，两者都有适应的纬度跃变。通过长期杂交实验，研究了光周期变异性与昼夜节律之间可能的共同遗传基础。将滞育临界日长较长的北方品系(65°N)与具有较短的滞育临界日长(CDL=19.9h)、早期携带节律的LD3：21(ψLD3：21=12.3h)、较短的自由奔跑节律(τ=18.8h)的南方品系(42°N)进行杂交。南方品系具有较短的CDL(12.4h)、羽化后期(ψLD3：21=20.2h)和较长的周期(τ=22.8h)。经过54代的自由重组、人工选择和遗传漂移，产生了一个新的品系，它比任何地理品系都具有更多的“南”滞育和更多的“北”羽化节律特征。观察到的羽化节律特征从光周期中完全分离是一项新发现，在跟踪16代的早期研究中，这些变化大多是平行的。显然，控制斜纹夜蛾羽化节律和光周期可变性的基因是不同的，但密切相关。为了测试可能的基因座潜在的观察到的地理变异性，研究了10个菌株的周期基因，涵盖了所有已知的钟型变异性。作者对最可疑的Thr-Gly区域进行了测序，已知该区域参与了果蝇的适应性时钟变异性。不同品系间没有发现编码差异，表明该区域不包括在小卷蛾的适应性时钟变异性中。
Drosophila littoralis is a latitudinally widespread European species of the Drosophila virilis group. The species has ample genetic variation in photoperiodism (adult diapause) and circadian rhythmicity (pupal eclosion rhythm), with adaptive latitudinal clines in both of them. The possible common genetic basis between the variability of photoperiodism and circadian rhythms was studied by a long-term crossing experiment. A northern strain (65 °N) having long critical day length (CDL = 19.9 h) for diapause, early phase of the entrained rhythm in LD 3:21 (ψLD3:21 = 12.3 h), and short period (τ= 18.8 h) of the free-running rhythm for the eclosion rhythm was crossed with a southern strain (42 °N) having short CDL (12.4 h), late eclosion phase (ψLD3:21 = 20.2 h), and long period (τ= 22.8 h). After 54 generations, including free recombination, artificial selection, and genetic drift, a novel strain resulted, having even more “southern” diapause and more “northern” eclosion rhythm characteristics than found in any of the geographical strains. The observed complete separation of eclosion rhythm characteristics from photoperiodism is a new finding in D. littoralis; in earlier studies followed for 16 generations, the changes had been mostly parallel. Evidently, the genes controlling the variability of the eclosion rhythm and photoperiodism in D. littoralis are different but closely linked. To test for the possible gene loci underlying the observed geographical variability, the period gene was studied in 10 strains covering all the known clock variability in D. littoralis. The authors sequenced the most suspected Thr-Gly region, which is known to take part in the adaptive clock variability in Drosophila melanogaster. No coding differences were found in the strains, showing that this region is not included in the adaptive clock variability in D. littoralis.
DOI：
10.1073/pnas.86.10.3748
发表时间：
1989-05-01
影响因子：
11.1
作者：
SAUNDERS, DS;HENRICH, VC;GILBERT, LI
通讯作者：
GILBERT, LI