DNA separation by capillary electrophoresis with hydrophilic substituted celluloses as coating and sieving polymers. Application to the analysis of genetically modified meals
DNA separation by capillary electrophoresis with hydrophilic substituted celluloses as coating and sieving polymers. Application to the analysis of genetically modified meals
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DOI：
10.1002/jssc.200401797
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发表时间：
2004-12-01
影响因子：
3.1
通讯作者：
Vanni, A
中科院分区：
文献类型：
--
作者：
Giovannoli, C;Anfossi, L;Vanni, A
基于羟丙基纤维素在毛细管柱壁上的物理吸附的涂布程序已成功地用于分离高达500 bp的DNA片段。该方法使用含有2-羟乙基纤维素的运行Tris-磷酸盐-EDTA缓冲液作为筛分聚合物。分离程序显示出良好的重现性（以RSD%测量），连续运行（
A coating procedure based on the physical adsorption of hydroxypropyl cellulose onto the wall of a capillary column has been successfully used for the separation of DNA fragments up to 500 bp. The method uses a running Tris-phosphate-EDTA buffer containing 2-hydroxyethyl cellulose as sieving polymer. The separation procedure shows good reproducibility (measured as RSD%) for consecutive runs (