RECEPTOR-MEDIATED TRANSPORT OF INSULIN ACROSS ENDOTHELIAL-CELLS
RECEPTOR-MEDIATED TRANSPORT OF INSULIN ACROSS ENDOTHELIAL-CELLS
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DOI：
10.1126/science.3883490
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
JOHNSON, SM
中科院分区：
文献类型：
--
作者：
KING, GL;JOHNSON, SM
胰岛素等激素从血管内部输送到血管外部。排列在血管内壁的内皮细胞是否在激素的这种运输中起作用尚不清楚，但已知内皮细胞可以迅速内化和释放胰岛素，几乎没有降解。125I标记的胰岛素的运输直接通过使用由水平单层培养的牛主动脉内皮细胞分隔的双室来测量。在这种情况下，内皮细胞吸收并释放标记的胰岛素，从而将其运送到细胞之间。胰岛素通过内皮细胞的转运是温度敏感的，并且受到未标记胰岛素和胰岛素受体抗体的抑制，与这些物质抑制胰岛素与其受体结合的能力成比例。超过80%的转运胰岛素是完整的。胰岛素显然通过受体介导的过程快速转运穿过内皮细胞。
Hormones such as insulin are transported from the interior to the exterior of blood vessels. Whether endothelial cells, which line the inner walls of blood vessels have a role in this transport of hormones is not clear, but it is known that endothelial cells can internalize and release insulin rapidly with little degradation. The transport of 125I-labeled insulin was measured directly through the use of dual chambers separated by a horizontal monolayer of cultured bovine aortic endothelial cells. In this setting, endothelial cells took up and released the labeled insulin, thereby transporting it across the cells. The transport of insulin across the endothelial cells was temperature sensitive and was inhibited by unlabeled insulin and by antibody to insulin receptor in proportion to the ability of these substances to inhibit insulin binding to its receptor. More than 80% of the transported insulin was intact. Insulin evidently is rapidly transported across endothelial cells by a receptor-mediated process.