A New Minimally Invasive Method of Transverse Aortic Constriction in Mice
A New Minimally Invasive Method of Transverse Aortic Constriction in Mice
复制标题
一种新型微创小鼠主动脉横缩术
DOI：
10.1007/s12265-021-10170-4
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发表时间：
2021-09-08
影响因子：
3.4
通讯作者：
Wu, Zhongkai
中科院分区：
文献类型：
--
作者：
Li, Huayang;Liu, Quan;Wu, Zhongkai
横断主动脉收缩（TAC）是小鼠最常用的模拟压力过载心脏病的模型。在本研究中，我们开发了一种方便、快速、微创的TAC小鼠新模型。简单地说，麻醉后气管插管，气管插管连接呼吸机。打开第二肋间隙，用自制牵开器轻轻将胸腺推到一边。在主动脉弓下建立一个隧道，将一段6-0单丝聚丙烯缝线穿过一个专门改良的钝26号注射器针头穿过隧道。一根27号的钝针与横主动脉平行，然后迅速打了三个结。结扎后，迅速轻柔地取出间隔器，使直径缩小0.4 mm。TAC后5周，观察到心肌肥厚、纤维化和左心室功能障碍。图示：采用戊巴比妥50 mg/kg腹腔注射麻醉小鼠。然后在直视下进行气管插管，气管插管连接呼吸机。打开第二肋间隙，用自制牵开器轻轻将胸腺推到一边。在主动脉弓下建立一个隧道，将一段6-0单丝聚丙烯缝线穿过一个专门改良的钝26号注射器针头穿过隧道。
Transverse aortic constriction (TAC) in mice is the most popular model to mimic pressure overload heart disease. In this study, we developed a convenient, quick, and less invasive new TAC mice model. Briefly, after anesthetization, endotracheal intubation was then performed, and the endotracheal tube was connected to a ventilator. The second intercostal space was opened and then the home-made retractors were used to push aside the thymus gently. A tunnel under the aortic arch was made and a segment of 6–0 monofilament polypropylene suture which had been threaded through a specifically modified blunted 26-gauge syringe needle was passed through the tunnel. A blunted 27-gauge needle was placed parallel to the transverse aorta and then three knots were tied quickly. After ligation, the spacer was removed promptly and gently to achieve a constriction of 0.4 mm in diameter. Five weeks after TAC, cardiac hypertrophy, fibrosis, and left ventricular dysfunction were observed.Graphical abstractThe mouse was anesthetized with pentobarbital (50 mg/kg) via intraperitoneal injection. Endotracheal intubation under direct vision was then performed and the endotracheal tube was connected to a ventilator. The second intercostal space was opened and then the home-made retractors were used to push aside the thymus gently. A tunnel under the aortic arch was made and a segment of 6–0 monofilament polypropylene suture which had been threaded through a specifically modified blunted 26-gauge syringe needle was passed through the tunnel.