Hematin is an alternative catalyst to horseradish peroxidase for in situ hydrogelation of polymers with phenolic hydroxyl groups in vivo.
Hematin is an alternative catalyst to horseradish peroxidase for in situ hydrogelation of polymers with phenolic hydroxyl groups in vivo.
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DOI：
10.1021/bm100623k
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发表时间：
2010-07
影响因子：
6.2
通讯作者：
S. Sakai;Kousuke Moriyama;K. Taguchi;K. Kawakami
中科院分区：
文献类型：
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作者：
S. Sakai;Kousuke Moriyama;K. Taguchi;K. Kawakami
血红素，含铁的卟啉用于管理卟啉病的攻击，被评估为辣根过氧化物酶（HRP）的替代催化剂，用于在体内与酚羟基（Ph）部分的聚合物的原位凝胶化。明胶衍生物与Ph部分的水溶液是可胶凝的血红素和H2O2的存在下。接种后4 h，L929成纤维细胞的粘附率为98.6%，其形态与未改性明胶涂层基质上的细胞相似，表明无明显的基质细胞毒性。在20 s内诱导凝胶化的条件下，凝胶化过程的高细胞相容性通过体外封闭细胞的95.0%活力来证明。此外，通过对原位形成的凝胶周围皮肤组织的组织学观察，与HRP相比，未发现血红素的不良反应。通过羧甲基纤维素衍生物的凝胶化证明了血红素用于具有Ph基团的各种聚合物的凝胶化的多功能性。
Hematin, an iron-containing porphyrin used in the management of porphyria attacks, was evaluated as an alternative catalyst to horseradish peroxidase (HRP) for in situ gelation of polymers with phenolic hydroxyl (Ph) moieties in vivo. An aqueous solution of gelatin derivative with Ph moieties was gellable in the presence of both hematin and H2O2. A total of 98.6% adhesion of L929 fibroblast cells 4 h after seeding and their similar morphology to those on substrate coated with unmodified gelatin indicated no obvious substrate cytotoxicity. High cytocompatibility of the gelation process under conditions inducing gelation within 20 s was demonstrated by 95.0% viability of enclosed cells in vitro. Furthermore, no adverse effects of hematin were found compared with HRP by histological observation of cutaneous tissue surrounding in situ formed gels. The versatility of hematin for gelation of a variety of polymers possessing Ph groups was demonstrated by the gelation of a carboxymethyl cellulose derivative.