Separation of membrane currents using a Paramecium mutant
Separation of membrane currents using a Paramecium mutant
复制标题
使用草履虫突变体分离膜电流
作者：
D. Oertel;S. Schein;C. Kung
使用电压钳技术测量了逐步去极化期间草履虫电可兴奋膜的净膜电流。突变体 pawn B 缺乏功能性 Ca 通道。因此，在野生型中测量的总电流与在pawn突变体中测量的漏电流和整流电流之间的差值是Ca电流。这些发现通过离子取代实验得到了证实。消除了失活可能仅是明显的并且由假设的叠加的 Ca 诱导的整流 K+ 电流引起的可能性。
The net membrane current of the electrically excitable membrane of Paramecium during step depolarisations was measured using voltage-clamp techniques. The mutant, pawn B, lacks a functional Ca channel. Thus the difference between the total current measured in the wild type and the leakage and rectification currents measured in the pawn mutant is the Ca current. These findings were confirmed by ion substitution experiments. The possibility that inactivation may be apparent only, and caused by a hypothetical, superposed, Ca-induced, rectifying K+ current was eliminated.