The mast cell products histamine and serotonin stimulate and TNF-α inhibits the proliferation of murine epidermal keratinocytes in situ
The mast cell products histamine and serotonin stimulate and TNF-α inhibits the proliferation of murine epidermal keratinocytes in situ
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DOI：
10.1016/s0923-1811(97)00043-1
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发表时间：
1997-11-01
影响因子：
4.6
通讯作者：
Paus, R
中科院分区：
文献类型：
--
作者：
Maurer, M;Opitz, M;Paus, R
为了更好地表征肥大细胞及其产物在调节表皮稳态中的生长调节功能，我们在小鼠皮肤器官培养物中加入[H-3]胸腺嘧啶，在不同浓度的组胺、5 -羟色胺或tnf - α孵育后，评估了角化细胞的增殖。与对照组相比，组胺和血清素都能显著刺激角质形成细胞的增殖（P < 0.001），在微至纳摩尔浓度范围内达到最大值。即使在亚纳摩尔浓度下，tnf - α也能显著抑制小鼠皮肤器官培养中表皮角质形成细胞对胸腺嘧啶的掺入（P < 0.001）。这些观察结果支持肥大细胞产物作为角化细胞增殖调节剂的概念，并提出肥大细胞在生理条件下调节表皮组织更新的作用。(C) 1997爱思唯尔科学爱尔兰有限公司
To better characterize the proposed growth modulatory functions of mast cells and their products in the regulation of epidermal homeostasis, we have assessed keratinocyte proliferation by incorporation of [H-3]thymidine in murine skin organ cultures after incubation with different concentrations of histamine, serotonin or TNF-alpha. Both histamine and serotonin significantly stimulate the proliferation of keratinocytes as compared to controls (P < 0.001), reaching maxima within a range from micro-to nanomolar concentrations. Even at subnanomolar concentration, TNF-alpha significantly inhibits the incorporation of labelled thymidine by epidermal keratinocytes in murine skin organ culture (P < 0.001). These observations support the concept of mast cell products as modulators of keratinocyte proliferation, and suggest a role for mast cells in the regulation of epidermal tissue turnover under physiological conditions. (C) 1997 Elsevier Science Ireland Ltd.