VISCERAL AND SOMATIC AFFERENT CONVERGENCE ONTO NEURONS NEAR THE CENTRAL CANAL IN THE SACRAL SPINAL-CORD OF THE CAT
VISCERAL AND SOMATIC AFFERENT CONVERGENCE ONTO NEURONS NEAR THE CENTRAL CANAL IN THE SACRAL SPINAL-CORD OF THE CAT
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DOI：
10.1152/jn.1985.53.4.1059
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发表时间：
1985-01-01
影响因子：
2.5
通讯作者：
HONDA, CN
中科院分区：
文献类型：
--
作者：
HONDA, CN
本文研究了30只风速去大脑猫的160个细胞外和细胞内记录的脊髓节段的单位放电，以观察躯体和内脏传入刺激对中央管附近神经元的影响。记录的单位活动经组织学证实(通过染色标记或辣根过氧化物酶，HRP)来自CC周围的灰质，接近Rexed的X板层。在没有刻意刺激或记录电极明显损伤的情况下，62%的单位表现为持续放电。测试每个单位对躯体(皮肤和皮下)和内脏(膀胱和结肠)结构刺激的反应。76个单位(48%)只对躯体感受野刺激有反应，10个单位(6%)对刺激盆腔内脏有选择性反应。其余74例(46%)的活动受躯体和内脏传入纤维活动的影响。160个神经元中有18个用HRP标记。根据细胞膜的大小和树突的范围，可以将这些细胞分为两个部分重叠的组。其中一组由7个神经元组成，这些神经元有小到中等大小的核周膜，树状乔木主要局限于CC周围的灰质，以及小的、单一的体细胞感受区。第二组由11个细胞组成，其胞体和树突从X板层延伸出来。这些较大的神经元通常具有多个广泛分布的体细胞感受野。本研究的主要发现是，在骶髓CC附近的许多细胞接受来自躯体和内脏结构中各种受体类型的初级传入输入。这种神经元能够整合来自身体两侧躯体结构的传入信息，以及起源于盆腔内脏和中线区域(如生殖器)的信息。
One hundred and sixty extracellularly and intracellularly recorded unitary discharges from the sacral or caudal spinal segments of 30 anemically decerebrated cats were studied to examine the effects of somatic and visceral afferent stimulation on neurons near the central canal (CC). The recorded unitary activity was histologically verified (by dye marks or horseradish peroxidase, HRP) as having come from the gray matter surrounding the CC that approximates Rexed's lamina X. In the absence of intentional stimulation or apparent injury by the recording electrode, 62% of the units exhibited ongoing discharges. Each unit was tested for responses to the stimulation of somatic (cutaneous and subcutaneous) and visceral (bladder and colon) structures. Seventy-six (48%) of the units responded exclusively to the stimulation of somatic receptive fields, and 10 (6%) of the units were selectively responsive to stimulation of the pelvic viscera. The activity of the remaining 74 (46%) was influenced by activity in both somatic and visceral afferent fibers. Eighteen of the 160 neurons were intracellularly marked with HRP. Based on perikaryal size and dendritic extent, it was possible to divide these cells into two partially overlapping groups. One group consisted of seven neurons with small to medium-sized perikarya, dendritic arbors largely restricted to the gray matter surrounding the CC, and small, singular somatic receptive fields. The second group comprised 11 cells with medium to large-sized soma and dendrites extending out of lamina X. These larger neurons usually possessed multiple, widely distributed somatic receptive fields. The principal finding of the present study is that in the sacral spinal cord many cells near the CC receive primary afferent inputs converging from a wide range of receptor types in somatic and visceral structures. Such neurons are capable of integrating afferent information from somatic structures on both sides of the body with information originating in pelvic viscera and midline regions such as the genitals.