TOXOPLASMOSIS IN IMMUNOGLOBULIN-M-SUPPRESSED MICE
TOXOPLASMOSIS IN IMMUNOGLOBULIN-M-SUPPRESSED MICE
复制标题
DOI：
10.1128/iai.38.1.360-367.1982
复制
发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
TAYLOR, DW
中科院分区：
文献类型：
--
作者：
FRENKEL, JK;TAYLOR, DW
用弓形虫致病株攻击的小鼠发生致命感染。如果这些小鼠最初用磺胺嘧啶（SD）治疗，它们会产生免疫力并在慢性感染中存活。使用B细胞缺陷（IgM抑制）、T细胞缺陷（无胸腺）和正常BALB/c小鼠研究了抗体（Ab）在建立针对急性寄生虫血症的保护性免疫和维持慢性感染中的作用。所有未接受SD治疗的小鼠在感染后迅速死亡（平均7.5天），但大多数（80%）完整小鼠在SD治疗期间产生免疫力并存活超过5个月。患有慢性弓形虫病无胸腺小鼠在SD处理停止后迅速死亡（平均6.0天）。尽管所有SD处理的IgM抑制小鼠最终死亡，但它们在完全不存在抗弓形虫抗体的情况下比未保护的小鼠（7-9天）存活得更长（18-83天）。肝、肺、脑和其他组织的组织病理学切片显示，弓形虫生物体在所有死亡动物中引起致命性病变。将Ab注射到急性感染和无胸腺的小鼠中不产生保护作用，但在SD治疗后将抗弓形虫Ab（滴度> 1：8000）转移到IgM抑制的小鼠中导致50%的小鼠中的寄生虫消除。显然，抗体在急性感染中可能不是决定性的，但在控制长期弓形虫病中可能很重要。
Mice challenged with a pathogenic strain of Toxoplasm gondii develop fatal infections. If such mice are initially treated with sulfadiazine (SD), they develop immunity and survive with chronic infections. The role of antibody (Ab) in establishing protective immunity against acute parasitemias and in maintaining chronic infections was investigated using B-cell-deficient (IgM-suppressed), T-cell-deficient (athymic), and normal BALB/c mice. All mice not receiving SD treatment rapidly died (mean 7.5 days) after infection, but the majority (80%) of intact mice developed immunity during SD treatment and survived for over 5 mo. with chronic toxoplasmosis. Athymic mice rapidly died (mean 6.0 days) after the removal of SD treatment. Although all SD-treated IgM-suppressed mice eventually died, they lived considerably longer (18-83 days) in the complete absence of antitoxoplasma Ab than unprotected mice (7-9 days). Histopathological sections of liver, lung, brain and other tissues showed that toxoplasma organisms gave rise to fatal lesions in all nonsurviving animals. The injection of Ab into acutely infected and athymic mice imparted no protection, but transfer of antitoxoplasma Ab (titer > 1:8000) to IgM-suppressed mice after SD treatment resulted in elimination of the parasites in 50% of the mice. Apparently, Ab may not be decisive in acute infections, but may be important in controlling long-term toxoplasmosis.