P-selectin expression, but not GPIIb/IIIa activation, is enhanced in the inflammatory stage of Takayasu's arteritis
P-selectin expression, but not GPIIb/IIIa activation, is enhanced in the inflammatory stage of Takayasu's arteritis
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DOI：
10.1253/circj.70.600
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发表时间：
2006-05-01
影响因子：
3.3
通讯作者：
Numano, F
中科院分区：
文献类型：
--
作者：
Kasuya, N;Kishi, Y;Numano, F
背景炎症和血栓形成是密切相关的过程，但在大动脉炎（TA）的疾病活动性和血栓形成之间的联系知之甚少。为了研究血小板活化与疾病活动性之间的联系，在TA患者中进行血小板P-选择素和活化GPHb/IIIa表达的流式细胞术分析。（A组，n=9）和非活动性根据血液来源的炎症标志物，研究了13名患者（I组，n=13）和14名年龄和性别匹配的健康对照（C组）。与C组相比，A组P-选择素对0.1-10 μ mol/L ADP的平均荧光强度显著上调，而I组则无明显变化。3组血小板GPIIb/IIIa表达无差异。标准的血小板聚集研究表明，疾病活动并不影响血小板聚集的ADP。结论P-选择素的表达，但不激活GPIIb/IIIa，增强ADP激活的血小板在TA的炎症阶段的患者。P-选择素可能通过诱导血小板-白细胞相互作用，在与难治性TA相关的炎症和血栓形成反应中发挥重要作用。
Background Inflammation and thrombosis are closely related processes, but the association between disease activity and thrombogenicity in Takayasu's arteritis (TA) is poorly understood. To investigate the link between platelet activation and disease activity, flow cytometric analyses of platelet P-selectin and activated GPHb/IIIa expression were performed in patients with TA.Methods and Results Twenty-two patients with TA, classified into active (Group A, n=9) and inactive (Group I, n=13) according to blood-derived inflammatory markers, and 14 healthy age- and gender-matched controls (Group C) were studied. Compared with Group C, the mean fluorescence intensity of P-selectin in response to 0.1-10 mu mol/L of ADP was significantly upregulated in Group A, but not in Group I. No differences in platelet GPIIb/IIIa expression in stimulated platelets were seen among the 3 groups. Standard platelet aggregation studies revealed that disease activity did not influence platelet aggregation by ADP.Conclusions P-selectin expression, but not activated GPIIb/IIIa, is enhanced in ADP-activated platelets from patients in the inflammatory stage of TA. P-selectin may play a significant role in the inflammatory and thrombotic responses associated with intractable TA, presumably by inducing platelet-leukocyte interactions.