Katanin's severing activity favors bundling of cortical microtubules in plants
Katanin's severing activity favors bundling of cortical microtubules in plants
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DOI：
10.1111/j.1365-313x.2006.02761.x
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发表时间：
2006-06-01
期刊：
影响因子：
7.2
通讯作者：
Vantard, Marylin
中科院分区：
文献类型：
--
作者：
Stoppin-Mellet, Virginie;Gaillard, Jeremie;Vantard, Marylin
高等植物细胞具有植物特有的间期微管阵列，这对它们的发育程序是必不可少的。这些皮层微管（CMT）由一群高度动态的微管组成，这些微管通常在细胞的皮层中组织成束。CMT的组织与专业化功能的获得密切相关，其分布的后续变化影响其属性。CMT形成和分布的机制尚不清楚，对参与这种现象的蛋白质知之甚少。在这里，我们调查了推定的作用katanin，唯一已知的植物微管切断蛋白，在组织的CMT。我们产生了在乙醇诱导型启动子控制下过表达katanin的转基因拟南芥品系。为了响应诱导的katanin过度表达，CMT组织成许多和厚束，最终解聚。从CMT模式的分析，再加上最近的数据CMT动力学，我们提出，在间期细胞，katanin的主要活动是免费的CMT，产生能动的微管，纳入束。
Higher plant cells exhibit interphase microtubule arrays specific to plants, which are essential for their developmental program. These cortical microtubules (CMT) consist of a population of highly dynamic microtubules that are usually organized into bundles in the cortex of the cells. The organization of CMT is intimately linked to the acquisition of specialized functions, and subsequent changes in their distribution affect their properties. The mechanisms underlying the formation and the distribution of CMT are still unclear, and little is known about the proteins that are involved in this phenomenon. Here we investigated the putative role of katanin, the only known plant microtubule-severing protein, in the organization of CMT. We generated transgenic Arabidopsis lines that overexpress katanin under the control of an ethanol-inducible promoter. In response to an induced overexpression of katanin, CMT organized into numerous and thick bundles, which ultimately depolymerized. From the analyses of CMT patterns together with recent data on CMT dynamics, we propose that, in interphase cells, katanin's main activity is to free CMT, generating motile microtubules that incorporate into bundles.