The Arabidopsis SUMO E3 ligase AtMMS21, a homologue of NSE2/MMS21, regulates cell proliferation in the root
The Arabidopsis SUMO E3 ligase AtMMS21, a homologue of NSE2/MMS21, regulates cell proliferation in the root
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拟南芥 SUMO E3 连接酶 AtMMS21 是 NSE2/MMS21 的同源物，调节根部细胞增殖
DOI：
10.1111/j.1365-313x.2009.03992.x
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发表时间：
2009-11-01
期刊：
影响因子：
7.2
通讯作者：
Yang, Chengwei
中科院分区：
文献类型：
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作者：
Huang, Lixia;Yang, Songguang;Yang, Chengwei
hMMS 21是预防DNA损伤诱导的细胞凋亡所需的SUMO E3连接酶，并通过促进人类细胞中的DNA修复而起作用。拟南芥基因组中含有一个推定的MMS 21同源物，能够与SUMO E2结合酶AtSCE 1a相互作用，如酵母双杂交筛选和双分子荧光互补实验所示。体外和体内实验结果表明AtMMS 21是一种SUMO E3连接酶。我们确定了拟南芥AtMMS 21无效T-DNA插入突变体MMS 21 -1，它具有短根表型，并影响根尖分生组织中的细胞增殖，如细胞分裂标记CYCB 1：GUS在MMS 21 -1根中的表达受损所示。与野生型相比，mms 21 -1根对外源细胞分裂素的反应降低，细胞分裂素诱导的基因ARR 3、ARR 4、ARR 5和ARR 7的表达降低。因此，我们的研究结果表明，AtMMS 21基因参与根的发展，通过细胞周期调控和细胞分裂素信号。
P>hMMS21 is a SUMO E3 ligase required for the prevention of DNA damage-induced apoptosis, and acts by facilitating DNA repair in human cells. The Arabidopsis genome contains a putative MMS21 homologue capable of interacting with the SUMO E2 conjugating enzyme AtSCE1a, as indicated by a yeast two-hybrid screen and bimolecular fluorescence complementation experiments. In vitro and in vivo data demonstrated that AtMMS21 was a SUMO E3 ligase. We identified the Arabidopsis AtMMS21 null T-DNA insertion mutant mms21-1, which had a short-root phenotype, and affected cell proliferation in the apical root meristem, as indicated by impaired expression of the cell division marker CYCB1:GUS in mms21-1 roots. The mms21-1 roots had reduced responses to exogenous cytokinins, and decreased expression of the cytokinin-induced genes ARR3, ARR4, ARR5 and ARR7, compared with the wild type. Thus, our findings suggest that the AtMMS21 gene is involved in root development via cell-cycle regulation and cytokinin signalling.