Electroporation of dissociated hippocampal neurons
Electroporation of dissociated hippocampal neurons
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分离海马神经元的电穿孔
DOI：
10.1007/978-1-4939-2459-2_13
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Tetsuichiro Saito
中科院分区：
文献类型：
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作者：
Atsushi Baba;Tetsuichiro Saito
一致和有效的基因转移到有丝分裂后神经元是研究神经元所需要的。最近细胞培养技术的改进使我们能够在分离培养中制备各种类型的神经元。其中，海马神经元在电镀后迅速极化，形成复杂的形态，在许多研究中都有很大的应用。虽然海马体神经元的祖细胞可以通过子宫内电穿孔有效地转染，但有丝分裂后的神经元比祖细胞更不容易被转染。我们已经开发出一种体外电穿孔方法，它允许快速和容易地转染到分离的海马神经元。
Consistent and efficient gene transfer into postmitotic neurons is desirable for the study of neurons. Recent refinement of cell culturing techniques has enabled us to prepare various types of neurons in dissociated cultures. Among them, hippocampal neurons rapidly polarize, form complex morphology after plating, and are of great use in many studies. Although progenitors of hippocampal neurons can be efficiently transfected by in utero electroporation, postmitotic neurons are less susceptible to transfection than progenitors. We have developed an in vitro electroporation method, which allows for quick and easy transfection into dissociated hippocampal neurons.