VERSICAN GENE-EXPRESSION IN HUMAN ARTICULAR-CARTILAGE AND COMPARISON OF MESSENGER-RNA SPLICING VARIATION WITH AGGRECAN
VERSICAN GENE-EXPRESSION IN HUMAN ARTICULAR-CARTILAGE AND COMPARISON OF MESSENGER-RNA SPLICING VARIATION WITH AGGRECAN
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DOI：
10.1042/bj2910361
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发表时间：
1993-04-15
影响因子：
4.1
通讯作者：
ROUGHLEY, PJ
中科院分区：
文献类型：
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作者：
GROVER, J;ROUGHLEY, PJ
所有年龄段的人关节软骨软骨细胞均表达聚集蛋白聚糖aggrecan和versican的mRNA，尽管versican mRNA的表达水平远低于aggrecan mRNA。Aggrecan在其c端球状区域显示表皮生长因子（EGF）样结构域的选择性剪接，但没有证据表明该结构域的相对原位表达随年龄的变化有重大差异。在从出生到成年的所有年龄段的研究中，更大比例的转录本缺乏EGF结构域。两种转录本的相对比例在培养和传代分离软骨细胞时没有改变。相反，邻近的补体调节蛋白（CRP）样结构域与年龄无关，但细胞培养确实导致该结构域剪接的变化。Versican具有两个egf样结构域和一个crp样结构域，但在所有年龄，这三个结构域主要存在于所有转录本中。这种情况在软骨细胞培养和传代过程中持续存在。因此，与聚集蛋白不同的是，无论是在原位软骨中还是在培养的软骨细胞中，人类关节软骨表达的versican似乎都不会经历其c端球形区域的选择性剪接。
The chondrocytes in human articular cartilage from subjects of all ages express mRNAs for both of the aggregating proteoglycans aggrecan and versican, although the level of expression of versican mRNA is much lower than that of aggrecan mRNA. Aggrecan shows alternative splicing of the epidermal growth factor (EGF)-like domain within its C-terminal globular region, but there is no evidence for a major difference in situ in the relative expression of this domain with age. At all ages studied from birth to the mature adult, a greater proportion of transcripts lacked the EGF domain. The relative proportions of the two transcripts did not change upon culture and passage of isolated chondrocytes. In contrast, the neighbouring complement regulatory protein (CRP)-like domain was predominantly expressed irrespective of age, but cell culture did result in variation of the splicing of this domain. Versican possesses two EGF-like domains and one CRP-like domain, but at all ages the three domains were predominantly present in all transcripts. This situation persisted upon culture and passage of the chondrocytes. Thus, unlike aggrecan, the versican expressed by human articular cartilage does not appear to undergo alternative splicing of its C-terminal globular region, either in cartilage in situ or in chondrocytes in culture.