EPSTEIN-BARR VIRUS (EBV)-CONTAINING NASOPHARYNGEAL CARCINOMA-CELLS EXPRESS THE B-CELL ACTIVATION ANTIGEN BLAST2 CD23 AND LOW-LEVELS OF THE EBV RECEPTOR CR-2
EPSTEIN-BARR VIRUS (EBV)-CONTAINING NASOPHARYNGEAL CARCINOMA-CELLS EXPRESS THE B-CELL ACTIVATION ANTIGEN BLAST2 CD23 AND LOW-LEVELS OF THE EBV RECEPTOR CR-2
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DOI：
10.1128/jvi.63.10.4121-4128.1989
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发表时间：
1989-10-01
影响因子：
5.4
通讯作者：
LENOIR, GM
中科院分区：
文献类型：
--
作者：
BILLAUD, M;BUSSON, P;LENOIR, GM
间变性鼻咽癌（NPC）细胞总是携带EB病毒（EBV）基因组，这是人类病毒相关癌症中唯一的关联。虽然EBV能够在上皮细胞中复制，但EBV受体（补体受体2型[CR2];也称为CD 21）在正常和恶性上皮细胞中表达的结果是相互矛盾的。我们在裸鼠中生长了五种不同的EBV相关NPC肿瘤，通过使用灵敏的转录测定，我们在这些细胞中检测到EBV受体CR2基因的非常弱的转录信号。这表明恶性鼻咽上皮细胞可能表达低水平的EBV受体。编码Blast 2/CD 23的基因在三个移植的NPC肿瘤中转录，Blast 2/CD 23是由EBV诱导的B细胞活化分子。在取自相同NPC细胞系的短期培养物的培养基中也检测到Blast 2/CD 23蛋白的可溶形式。在淋巴系统中，Blast 2/CD 23的表达与EB病毒核抗原（EBNA 2）的表达，相反，没有EBNA 2蛋白可以检测到这些NPC上皮细胞。我们的研究首次证明了Blast 2/CD 23在上皮细胞中的表达。由于Blast 2/CD 23蛋白的可溶性形式具有与EBV诱导的B细胞永生化相关的生长因子活性，这些结果表明该分子在NPC的发病机制中可能起作用。
Anaplastic nasopharyngeal carcinoma (NPC) cells invariably harbor the Epstein-Barr virus (EBV) genome, an association that is unique among human virus-associated cancers. Although EBV is able to replicate in epithelial cells, results with expression of the EBV receptor (complement receptor type 2 [CR2]; also called CD21) in normal and malignant epithelial cells are conflicting. We grew five different EBV-associated NPC tumors in nude mice, and by using a sensitive transcriptional assay, we detected a very weak transcription signal of the EBV receptor CR2 gene in these cells. This suggests that low levels of EBV receptor may be expressed by malignant epithelial nasopharyngeal cells. The gene coding for Blast2/CD23, a B-cell activation molecule induced by EBV, was transcribed in three of the transplanted NPC tumors. The soluble form of the Blast2/CD23 protein was also detected in medium taken from short-term cultures of the same NPC cell lines. In contrast to the lymphoid system, in which Blast2/CD23 expression is associated with EBV nuclear antigen (EBNA2) expression, no EBNA2 protein could be detected in these NPC epithelial cells. Our study represents the first demonstration of Blast2/CD23 expression in epithelial cells. As the soluble form of the Blast2/CD23 protein possesses growth factor activity associated with EBV-induced B-cell immortalization, these results suggest a possible role for this molecule in the pathogenesis of NPC.