SV40 recombinants carrying a functional RNA splice junction and polyadenylation site from the chromosomal mouse βmaj globin gene
SV40 recombinants carrying a functional RNA splice junction and polyadenylation site from the chromosomal mouse βmaj globin gene
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SV40 重组体携带来自小鼠染色体 βmaj 球蛋白基因的功能性 RNA 剪接点和多聚腺苷酸化位点
DOI：
10.1016/0092-8674(79)90280-0
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发表时间：
1979
期刊：
影响因子：
64.5
通讯作者：
P. Leder
中科院分区：
文献类型：
--
作者：
D. Hamer;P. Leder
我们将小鼠染色体上的小球蛋白基因片段导入到SV40中，并利用由此产生的杂交病毒感染培养的猴肾细胞。小鼠DNA片段包含一个介入序列和一个多聚(a)添加位点，已被插入到相对于SV40晚期区域启动子的两个可能方向上。而片段的转录与取向无关，只有当片段以“意义”取向插入时，RNA剪接信号和poly (A)添加位点才会被利用。因此，剪接和聚腺苷酸化的小鼠基因组信号是跨物种边界识别的。此外，由于在中间序列的5 '边界上只包含了一个18bp的片段，因此我们可以定义该剪接信号的最大长度。
We have introduced a fragment of the chromosomal mouse jYior globin gene into SV40 and used the resultant hybrid virus to infect cultured monkey kidney cells. The mouse DNA fragment, which contains an intervening sequence and a poly (A) addition site, has been inserted in both possible orientations relative to the SV40 late region promoter. While the fragment is transcribed regardless of orientation, the RNA splice signal and poly (A) addition site are utilized only when the fragment is inserted in the “sense” orientation. Thus genomic mouse signals for both splicing and polyadenylation are recognized across species boundaries. Furthermore, since only an 18 bp segment was included on the 5’border of the intervening sequence, we can define a maximum length for this splice signal.