Pharmacologic properties of isolated proximal pulmonary arteries after seven-day exposure to in vivo hyperoxia.
Pharmacologic properties of isolated proximal pulmonary arteries after seven-day exposure to in vivo hyperoxia.
复制标题
体内高氧暴露 7 天后离体近端肺动脉的药理学特性。
DOI：
10.1164/ajrccm/138.4.945
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Evans,JN
中科院分区：
文献类型：
--
作者：
Coflesky,JT;Evans,JN
内皮细胞的显着损伤与肺动脉高压有关，肺动脉高压是在常压下体内暴露于高草酸期间发生的。我们假设内皮细胞损伤可能通过改变血管活性化合物的代谢和/或调节血管对那些需要完整内皮来发挥作用的药物的反应，从而导致高血压发展过程中血管张力的初始增加。本研究报告了体内肺部高氧损伤对离体肺血管药理学特性的影响。使用肌动描记器技术研究了连续 7 天呼吸 85% 0 的成年大鼠和断奶大鼠的近端肺动脉。记录等长张力的发展，以响应前列腺素F，（PGF，）的累积添加，并随后评估乙酰胆碱（ACh）松弛预收缩血管的能力。对 PGF 的敏感性。相对于对照，成体和断奶仔高氧血管中的含量均有所增加。相反，高氧损伤后乙酰胆碱的松弛程度降低。对照血管在添加乙酰胆碱后完全松弛，而在成人高氧动脉中仅记录到30％的松弛，并且在断奶高氧组织中测量到50％的松弛。这种对血管舒张的作用是内皮依赖性扩张剂乙酰胆碱所特有的。相比之下，硝普钠和罂粟碱（内皮依赖性激动剂）的松弛反应在高氧血症后不受影响。
Marked damage to the endothelium Is associated with the pulmonary hypertension that develops during In vivo exposure to hyperoxla at normobaric pressures. We hypothesized that endothelial cell damage may contribute to Initial increases In vascular tone during the development of hypertension by altering the metabolism of vasoactive compounds and/or modulating vessel responses to those agents that requlre an Intact endothelium for their actions. This study reports the effects of In vivo hyperoxic damage to the lung on the pharmacologic properties of Isolated pulmonary vessels. Proximal pulmonary arteries isolated from adult and weanling rats that breathed85% 0, for 7 days were studied using myograph techniques. Isometric tension development was recorded In response to the cumulative addition of prostaglandin F,.(PGF,.) and the ability of acetylcholine (ACh) to relax precontracted vessels was subsequently assessed. Sensitivities to PGF,. were increased In both adult and weanling hyperoxic vessels relative to control. Conversely, relaxation to acetylcholine was reduced following hyperoxic injury. Control vessels relaxed completely to acetylcholine addition, while only a 30% relaxation was recorded In adult hyperoxlc arteries and a 50% relaxation was measured in weanling hyperoxic tissues. This effect on vasodilation was specific for the endothelium-dependent dilator ACh. By contrast, relaxation responses to sodium nitroprusside and papaverine, endothelium-independent agonist&, wereunaffected following hyperox-