The simultaneous determination of diazepam and its three metabolites in dog plasma by high-performance liquid chromatography with mass spectroscopy detection.
The simultaneous determination of diazepam and its three metabolites in dog plasma by high-performance liquid chromatography with mass spectroscopy detection.
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DOI：
10.1016/s0731-7085(01)00404-6
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发表时间：
2001-09
影响因子：
3.4
通讯作者：
Z. Liu;J. Short;A. Rose;S. Ren;N. Contel;S. Grossman;S. Unger
中科院分区：
文献类型：
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作者：
Z. Liu;J. Short;A. Rose;S. Ren;N. Contel;S. Grossman;S. Unger
建立了一种快速、灵敏、特异的LC/MS/MS同时测定犬血浆中地西泮及其三种代谢物恶西泮、替马西泮和去甲基地西泮的方法。该方法由96孔自动固相萃取和电喷雾LC/MS/MS分析组成。d5 -地西泮作为所有化合物的内标。日内、日间测定变异系数均小于12.7%。每一种分析物的定量下限（LLOQ）为1 nM，以0.1 ml等分狗血浆为基础。分析运行时间为5分钟。在1-500 nM范围内观察到线性。这种方法已被用于支持药代动力学研究的发现。
A fast, sensitive and specific LC/MS/MS method for the simultaneous determination of diazepam and its three metabolites, oxazepam, temazepam and desmethyldiazepam, in dog plasma is described. The method consists of an automated 96-well solid phase extraction procedure and electrospray LC/MS/MS analysis. D5-Diazepam is used as the internal standard for all the compounds. Intra-day and inter-day assay coefficients of variations are less than 12.7%. The lower limit of quantitation (LLOQ) is 1 nM for each analyte, based on 0.1 ml aliquots of dog plasma. The analytical run time was 5 min. Linearity is observed over the range of 1–500 nM. This method has been used to support the discovery of pharmacokinetic studies.