Safety of Autologous Bone Marrow Stromal Cell Transplantation in Dogs with Acute Spinal Cord Injury
Safety of Autologous Bone Marrow Stromal Cell Transplantation in Dogs with Acute Spinal Cord Injury
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DOI：
10.1111/j.1532-950x.2011.00959.x
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发表时间：
2012-05-01
影响因子：
1.8
通讯作者：
Inaba, Toshio
中科院分区：
文献类型：
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作者：
Nishida, Hidetaka;Nakayama, Masanari;Inaba, Toshio
目的探讨自体骨髓基质细胞（BMSC）移植治疗急性脊髓损伤（SCI）的可行性和安全性。研究设计：开放标签单组试验。动物犬（n = 7）T6至L5严重SCI，由椎骨骨折和脱位引起。方法对犬脊柱骨折脱位致严重脊髓损伤进行减压稳定手术。从每只狗的股骨中获得自体BMSCs，培养，然后在急性期将其注射到病变中。观察脊髓损伤后1年以上不良事件及运动和感觉功能。结果术后随访2962个月。未观察到并发症（例如感染、神经性疼痛、神经功能恶化）。2只犬在无支撑的情况下行走，但7只犬的感觉功能均无任何变化。结论自体骨髓间充质干细胞移植治疗急性脊髓损伤是安全可行的。需要进一步的研究来确定这种疗法的疗效。
Objective To assess the feasibility and safety of transplantation of autologous bone marrow stromal cell (BMSC) in dogs with acute spinal cord injury (SCI). Study Design An open-label single-arm trial. Animals Dogs (n = 7) with severe SCI from T6 to L5, caused by vertebral fracture and luxation. Methods Decompressive and stabilization surgery was performed on dogs with severe SCI caused by vertebral fracture and luxation. Autologous BMSCs were obtained from each dog's femur, cultured, and then injected into the lesion in the acute stage. Adverse events and motor and sensory function were observed for >1 year after SCI. Results Follow-up was 2962 months after SCI. No complications (eg, infection, neuropathic pain, worsening of neurologic function) were observed. Two dogs walked without support, but none of the 7 dogs had any change in sensory function. Conclusions Autologous BMSC transplantation is feasible and safe in dogs with acute SCI. Further studies are needed to determine the efficacy of this therapy.