Rapid focus reduction neutralization test of influenza A and B viruses in microtiter system
Rapid focus reduction neutralization test of influenza A and B viruses in microtiter system
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微量滴定系统中甲型和乙型流感病毒的快速焦点还原中和试验
DOI：
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发表时间：
1990
影响因子：
9.4
通讯作者：
S. Ueda
中科院分区：
文献类型：
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作者：
Y. Okuno;Koji Kariya;K. Baba;A. Maeda;N. Kunita;S. Ueda
研制了一种甲型和乙型流感病毒快速中和试验。该方法采用96孔组织培养板制备细胞单层，采用过氧化物酶-抗过氧化物酶染色技术对感染病毒的病灶进行可视化观察。在胰蛋白酶和黄甲胶存在的情况下，感染后1天出现清晰的灶。观察到病毒稀释度与疫源地数量呈线性关系。当对某些检测血清中的中和抗体进行检测时，观察到焦点法获得的滴度与普通斑块法获得的滴度具有良好的相关性。此外，通过中和试验对许多血清标本进行了调查，结果表明，该试验对流感的血清学研究是有用的。
A rapid neutralization test for influenza A and B viruses was developed. In this method, a 96-well tissue culture plate was used for the preparation of cell monolayers and the peroxidase-antiperoxidase staining technique was used for the visualization of foci infected with these viruses. In the presence of trypsin and tragacanth gum, clear foci developed 1 day after infection. A linear relationship between virus dilutions and numbers of foci was observed. When neutralizing antibodies in some test sera were assayed, a good correlation was observed between the titers obtained by the focus method and those obtained by the ordinary plaque method. In addition, many serum specimens were investigated by the neutralization test, and it was demonstrated that the test is useful for serological studies of influenza.
影响因子：
3.7
作者：
KIDA, H;BROWN, LE;WEBSTER, RG
通讯作者：
WEBSTER, RG