Artificial simulated saliva, gastric and intestinal digestion of polysaccharide from the seeds of Plantago asiatica L.
Artificial simulated saliva, gastric and intestinal digestion of polysaccharide from the seeds of Plantago asiatica L.
复制标题
人工模拟唾液、胃、肠消化车前子种子多糖。
DOI：
10.1016/j.carbpol.2012.10.072
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发表时间：
2013-02-15
影响因子：
11.2
通讯作者：
Xie, Ming-Yong
中科院分区：
文献类型：
--
作者：
Hu, Jie-Lun;Nie, Shao-Ping;Xie, Ming-Yong
对车前多糖的唾液、胃和小肠消化特性进行了研究。种子进行了体外研究。结果表明，唾液淀粉酶对多糖无明显影响，但在消化后期对多糖有一定影响。随着消化时间的增加，观察到多糖的分子量（M-w）从1903.1 +/- 93.0稳定降低至4.7 +/-0.2kDa。同时，还原末端从0.157 +/- 0.009增加到0.622 +/- 0.026 mM，表明M-w的降低可能是由于糖苷键的断裂。此外，在整个消化期间没有单糖释放，表明胃肠道消化不会导致游离单糖的产生。这些结果可为进一步研究积雪草多糖的酶解提供参考。在体外，并可能有助于研究其他碳水化合物的消化方法。(C)2012爱思唯尔有限公司保留所有权利。
The saliva, gastric and intestinal digestion of polysaccharide from Plantago asiatica L. seeds was investigated in vitro. It was found that salivary amylase had no effect on the polysaccharide; however, the polysaccharide was influenced in later gastrointestinal digestion. A steady decrease in molecular weight (M-w) of the polysaccharide from 1903.1 +/- 93.0 to 4.7 +/- 0.2 kDa was observed as digestion time increased. Meanwhile, the reducing ends were increased from 0.157 +/- 0.009 to 0.622 +/- 0.026 mM, indicating the decrease of M-w may due to the breakdown of glycosidic bonds. In addition, there was no monosaccharide released throughout the whole digestion period, suggesting that the gastrointestinal digestion did not result in a production of free monosaccharide. These results may provide some information on the digestion of polysaccharide from P. asiatica L. in vitro, and may contribute to the methods of studying the digestion of other carbohydrates. (C) 2012 Elsevier Ltd. All rights reserved.