Flow-through micro-electroporation chip for high efficiency single-cell genetic manipulation
Flow-through micro-electroporation chip for high efficiency single-cell genetic manipulation
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DOI：
10.1016/s0924-4247(03)00050-5
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发表时间：
2003-05-15
影响因子：
4.6
通讯作者：
Rubinsky, B
中科院分区：
文献类型：
--
作者：
Huang, Y;Rubinsky, B
单个细胞的基因操作在生物学和生物技术中引起了极大的兴趣。我们之前的工作已证明微电穿孔技术能够进行受控电穿孔以促进单个生物细胞中的基因转移。本文通过介绍改进的微电穿孔芯片的设计和制造来扩展工作，该芯片利用微流体通道以流通方式精确处理细胞，以实现细胞基因操作的高效性。还证明了大分子受控地引入单个细胞中。单个细胞的转染证明了单细胞基因工程的可行性。 (C) 2003 Elsevier Science B.V. 保留所有权利。
Genetic manipulation of individual cells is of great interests in biology and biotechnology. Micro-electroporation technology has been demonstrated in our previous work with the capability of performing controlled electroporation to facilitate gene transfer in individual biological cells. This paper extends the work by introducing the design and fabrication of an improved micro-electroporation chip that employs microfluidic channels to precisely handle cells in a flow-through manner to achieve high effectiveness in genetic manipulation of cells. Controlled introduction of macromolecules into individual cells are also demonstrated. Transfection of an individual cell is shown to prove the feasibility of single-cell genetic engineering. (C) 2003 Elsevier Science B.V. All rights reserved.