Quantitative analysis of elevated serum Golgi protein-73 expression in patients with liver diseases
Quantitative analysis of elevated serum Golgi protein-73 expression in patients with liver diseases
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DOI：
10.1258/acb.2008.008088
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发表时间：
2009-01-01
影响因子：
2.2
通讯作者：
Peng, Tao
中科院分区：
文献类型：
--
作者：
Gu, Yanli;Chen, Wenli;Peng, Tao
背景：高尔基体蛋白73（GP 73）是一种新发现的肝脏疾病的候选血清标志物。这种生物标志物的实用性仍然有限，主要是由于缺乏定量信息。本研究旨在检测健康人和肝病患者血清GP 73（sGP 73）水平，探讨sGP 73作为肝病早期诊断的生物标志物的价值。结果：肝病患者血清中sGP 73的中位数显著高于健康人和其他疾病患者（P < 0.001）;当sGP 73用于检测肝脏疾病时，在最佳截止值85.5 μ g/L时，其灵敏度为82%，特异性为80%。受试者工作特征曲线下面积为0.9。结论：肝病患者血清sGP 73浓度是健康人的3倍。然而，sGP 73浓度在每个肝病组的患者之间没有显著差异。此外，与健康个体相比，sGP 73在患有肝病以外的疾病的患者中没有显著升高。提示sGP 73可作为肝病诊断的血清标志物。
Background: Golgi protein-73 (GP73) is a newly identified candidate serum marker for liver diseases. The utility of this biomarker remains limited, largely due to the lack of quantitative information. The aims of this study were to quantify serum GP73 (sGP73) in healthy individuals and in patients with liver diseases, and to validate sGP73 as a biomarker for early diagnosis of liver disease.Methods: Recombinant GP73 was used to generate monoclonal (mAb) and polyclonal antibodies (pAb). Using these antibodies in a quantitative enzyme-linked immunosorbent assay, GP73 was measured in serum from 263 patients with various forms of liver and other diseases.Results: The median sGP73 in patients with liver disease was significantly higher (P < 0.001) than in healthy individuals and in patients with other diseases. When sGP73 was used to detect liver disease, it had a sensitivity of 82% and a specificity of 80% at the optimal cut-off value of 85.5 mu g/L. The area under the receiver-operating characteristic curve was 0.9.Conclusion: sGP73 concentration in patients with liver disease was three-fold higher than in healthy individuals. However, sGP73 concentrations did not differ significantly between patients from each liver disease group. Furthermore, sGP73 was not significantly elevated in patients with diseases other than liver disease compared with healthy individuals. These results suggest that sGP73 may be used as a serum marker for the diagnosis of liver disease.