Possible autocrine enkephalin regulation of catecholamine release in human pheochromocytoma cells
Possible autocrine enkephalin regulation of catecholamine release in human pheochromocytoma cells
复制标题
DOI：
10.1016/j.lfs.2008.07.004
复制
发表时间：
2008-09-12
期刊：
影响因子：
6.1
通讯作者：
Levin, Gloria
中科院分区：
文献类型：
--
作者：
Herminia Pellizzari, Eliana;Barontini, Marta;Levin, Gloria
目的：嗜铬细胞瘤是分泌儿茶酚胺的肿瘤，也合成和分泌几种神经肽，包括阿片类药物。在嗜铬细胞中，阿片类物质对儿茶酚胺分泌有负调节作用。然而，到目前为止，所获得的结果是矛盾的，当提到人类嗜铬细胞瘤。本研究的目的是确定本地产生的脑啡肽对人嗜铬细胞瘤细胞释放儿茶酚胺的作用。主要方法：从11个不同遗传来源的人嗜铬细胞瘤获得的细胞培养5天。培养物在基础条件下或在脑啡肽、地塞米松和纳洛酮单独或与脑啡肽或地塞米松刺激条件组合下维持。用高效液相色谱-电化学发光法和放射免疫法分别测定培养液中儿茶酚胺和脑啡肽的含量。地塞米松治疗，增加脑啡肽水平，也降低了儿茶酚胺水平。另一方面，除了纳洛酮的文化恢复到正常的脑啡肽和地塞米松treatments.Significance施加的抑制作用：这些结果表明，脑啡肽对人嗜铬细胞瘤细胞释放去甲肾上腺素的自分泌负调节环的存在。(c)2008年爱思唯尔公司All rights reserved.
Aims: Pheochromocytomas are catecholamine-secreting tumors that also synthesize and secrete several neuropeptides, including opioids. A negative regulation of catecholamine secretion by opioids has been postulated in chromaffin cells. However, results obtained so far are contradictory when referred to human pheochromocytomas. The aim of this study was to define the role of locally produced enkephalins on catecholamine release in human pheochromocytoma cells.Main methods: Cells obtained from eleven human pheochromocytomas of different genetic origins were cultured for 5 days. Cultures were maintained under basal condition or under enkephalin, dexamethasone and naloxone alone or in combination with enkephalin or dexamethasone-stimulated conditions. Catecholamine and enkephalin levels in the culture medium were measured by HPLC-ED and RIA respectively.Key findings: Enkephalin induced a decrease in norepinephrine levels in all tumor cultures. Dexamethasone treatment, which increased enkephalin levels, also decreased catecholamine levels. On the other hand, the addition of naloxone to the cultures reverted to normal the inhibitory action exerted by enkephalin and dexamethasone treatments.Significance: These results suggest the existence of an autocrine negative regulatory loop exerted by enkephalin on norepinephrine release in human pheochromocytoma cells. (c) 2008 Elsevier Inc. All rights reserved.