The equine herpesvirus 2 E1 open reading frame encodes a functional chemokine receptor
The equine herpesvirus 2 E1 open reading frame encodes a functional chemokine receptor
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DOI：
10.1128/jvi.73.12.9843-9848.1999
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发表时间：
1999-12-01
影响因子：
5.4
通讯作者：
Napolitano, M
中科院分区：
文献类型：
--
作者：
Camarda, G;Spinetti, G;Napolitano, M
几种疱疹病毒含有编码真核基因的潜在同源物的开放阅读框（ORF）。马疱疹病毒2型（EHV-2）是一种γ疱疹病毒，与其他嗜淋巴细胞疱疹病毒如松鼠猴疱疹病毒和爱泼斯坦-巴尔病毒相关。EHV-2的E1 ORF是一种G蛋白偶联受体同源物，与已知的CC趋化因子受体具有31%至47%的氨基酸同一性。为了研究E1是否可以编码功能性受体，我们克隆了E1 ORF并在稳定转染的细胞系中表达。我们在这里报告的CC趋化因子eotaxin作为EHV-2 E1受体的功能性配体的鉴定。在EHV-2感染期间，趋化因子可能在马宿主的免疫功能调节中发挥作用，并且通过与E1相互作用，可能影响病毒复制和/或逃避免疫应答。
Several herpesviruses contain open reading frames (ORFs) that encode potential homologs of eucaryotic genes. Equine herpesvirus 2 (EHV-2) is a gammaherpesvirus related to other lymphotropic herpesviruses such as herpesvirus saimiri and Epstein-Barr virus. The E1 ORF of EHV-2, a G protein-coupled receptor homolog, shows 31 to 47% amino acid identity with known CC chemokine receptors. To investigate whether E1 may encode a functional receptor, we cloned the E1 ORF and expressed it in stably transfected cell lines. We report here the identification of the CC chemokine eotaxin as a functional ligand for the EHV-2 E1 receptor. Chemokines are likely to play a role in the regulation of immune functions in equine hosts during EHV-2 infection and, via interaction with E1, may affect viral replication and/or escape from immune responses.