Aminoglycoside 2'-N-acetyltransferase genes are universally present in mycobacteria: Characterization of the aac(2')-lc gene from Mycobacterium tuberculosis and the aac(2')-ld gene from Mycobacterium smegmatis
Aminoglycoside 2'-N-acetyltransferase genes are universally present in mycobacteria: Characterization of the aac(2')-lc gene from Mycobacterium tuberculosis and the aac(2')-ld gene from Mycobacterium smegmatis
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DOI：
10.1046/j.1365-2958.1997.3471717.x
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发表时间：
1997-04-01
影响因子：
3.6
通讯作者：
Martin, C
中科院分区：
文献类型：
--
作者：
Ainsa, JA;Perez, E;Martin, C
分枝杆菌属包括临床上重要的病原体，如M.结核病已重新成为世界范围内发病和死亡的主要原因，特别是随着多药耐药菌株的出现。使用快速生长的物种，如耻垢分枝杆菌，使分枝杆菌遗传学领域和分枝杆菌耐药机制的研究取得了重要进展。最近有报道从偶发分枝杆菌中分离出氨基糖苷类耐药基因。aac（2 '）-lb基因是染色体编码的，并且存在于所有M.很偶然。在其他分枝杆菌物种中存在该基因的研究在这里和基因同源的M。在所研究的所有分枝杆菌物种中都发现了偶然性。本文报道了从黑曲霉中克隆aac（2 '）-lc基因。结核分枝杆菌H37 Rv和结核分枝杆菌aac（2 '）-ld基因。描述了smeglycemc（2）155。Southern印迹杂交表明，这两个基因都存在于该物种的所有菌株研究的日期。此外，推定的aac（2 '）-le基因已定位于最近公布的麻风分枝杆菌基因组中。aac（2 '）-lc和aac（2'）-ld基因的表达已经在M.而只有AAC（2 ′）-LD与氨基糖苷类耐药相关。为了阐明氨基糖苷类2 ′-N-乙酰基转移酶基因在分枝杆菌中的作用，并确定它们是否是沉默的耐药基因或是否在分枝杆菌代谢中起次要作用，从分枝杆菌中克隆了aac（2 ′）-ld基因。M.的染色体中的耻垢已被破坏。155.第一百五十五章：你是我的女人破坏剂显示氨基糖苷类敏感性增加，沿着对溶菌酶的敏感性略有增加。
The genus Mycobacterium comprises clinically important pathogens such as M. tuberculosis, which has reemerged as a major cause of morbidity and mortality world-wide especially with the emergence of multidrug-resistant strains. The use of fast-growing species such as Mycobacterium smegmatis has allowed important advances to be made in the field of mycobacterial genetics and in the study of the mechanisms of resistance in mycobacteria. The isolation of an aminoglycoside-resistance gene from Mycobacterium fortuitum has recently been described. The aac(2')-lb gene is chromosomally encoded and is present in all isolates of M. fortuitum. The presence of this gene in other mycobacterial species is studied here and genes homologous to that of M. fortuitum have been found in al mycobacterial species studied. In this report, the cloning of the aac(2')-lc gene from M. tuberculosis H37Rv and the aac(2')-ld gene from M. smegmatis mc(2)155 is described. Southern blot hybridizations have shown that both genes are present in all strains of this species studied to date. In addition, the putative aac(2')-le gene has been located in a recent release of the Mycobacterium leprae genome. The expression of the aac(2')-lc and aac(2')-ld genes has been studied in M. smegmatis and only aac(2')-ld is correlated with aminoglycoside resistance. In order to elucidate the role of the aminoglycoside 2'-N-acetyltransferase genes in mycobacteria and to determine whether they are silent resistance genes or whether they have a secondary role in mycobacterial metabolism, the aac(2')-ld gene from M. smegmatis has been disrupted in the chromosome of M. smegmatis mc(2)155. The disruptant shows an increase in aminoglycoside susceptibility along with a slight increase in the susceptibility to lysozyme.