Prediction of replication origins by calculating DNA structural properties
Prediction of replication origins by calculating DNA structural properties
复制标题
通过计算 DNA 结构特性预测复制起点
DOI：
10.1016/j.febslet.2012.02.034
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发表时间：
2012-03-23
期刊：
影响因子：
3.5
通讯作者：
Lin, Hao
中科院分区：
文献类型：
--
作者：
Chen, Wei;Feng, Pengmian;Lin, Hao
在这项研究中，我们引入了两个DNA结构特征，即弯曲性和羟基自由基切割强度分析复制起点（ORI）的酿酒酵母基因组。我们发现，在核心复制区的DNA弯曲性和切割强度显着低于在连接区。通过使用这两个DNA结构特征，我们开发了一个用于ORI预测的计算模型，并在基准数据集中评估了该模型。刀切交叉验证的预测性能表明，DNA的弯曲度和切割强度有能力描述核心复制区域，我们的模型是有效的ORI预测。(C)2012年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
In this study, we introduced two DNA structural characteristics, namely, bendability and hydroxyl radical cleavage intensity to analyze origin of replication (ORI) in the Saccharomyces cerevisiae genome. We found that both DNA bendability and cleavage intensity in core replication regions were significantly lower than in the linker regions. By using these two DNA structural characteristics, we developed a computational model for ORI prediction and evaluated the model in a benchmark dataset. The predictive performance of the jackknife cross-validation indicates that DNA bendability and cleavage intensity have the ability to describe core replication regions and our model is effective in ORI prediction. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.