Interleukin-6 is a growth factor for nonmalignant human plasmablasts
Interleukin-6 is a growth factor for nonmalignant human plasmablasts
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DOI：
10.1182/blood.v97.6.1817
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发表时间：
2001-03-15
期刊：
影响因子：
20.3
通讯作者：
Pellat-Deceunynck, C
中科院分区：
文献类型：
--
作者：
Jego, G;Bataille, R;Pellat-Deceunynck, C
白介素6(IL-6)虽然常被认为是B细胞分化因子，但最近被认为是反应性浆细胞增多症中体内人浆母细胞的基本生存因子。参与分化的细胞包括前浆母细胞(CD20(+/-)、CD38(+/-)、CD138(-))、浆母细胞(CD20(-)、CD38(++)、CD138(-))和早期浆细胞(CD20(-)、CD38(+)、CD138(+))。IL-2或IL-10均能诱导较强的浆母细胞和早期浆细胞(PC)的生成，与IL-2或IL-10相比，IL-6对PC的生成效率较低，但与IL-2或IL-10联合使用时，IL-6可促进早期PC的生成。此外，抗IL-6单抗可显著减少IL-2诱导的早期浆细胞的生成，但不能减少浆母细胞的生成。IL-2和IL-6的这些作用与它们各自受体(R)的表达差异是一致的，CD25(IL-2Rα)在活化的B细胞上增加72+/-10倍，而在浆母细胞上减少然后消失，相反，CD126(IL-6Rα)在活化的B细胞上几乎不表达，但在前浆母细胞上增加18+/-2倍，最后IL-6促进IL-2产生的浆母细胞的增殖(2倍增加)，数据表明IL-6是一种非恶性浆细胞的生长因子，(血液，2001；97：1817-1822)(C)2001，由美国血液病学会主办。
Interleukin-g (IL-6), although often regarded as a B-cell differentiation factor, was recently described as the essential survival factor for human plasmablasts in vivo in reactive plasmacytosis, The present study reinvestigated the roles of IL-6 and IL-2 in the generation of plasma cells from human memory B cells in vitro. The cells involved in this differentiation process were identified as preplasma-blasts (CD20(+/-)CD38(+/-)CD138(-)), plasmablasts (CD20(-)CD38(++)CD138(-)), and early plasma cells (CD20(-)CD38(+++)CD138(+++)). IL-2 or IL-10 induced a strong generation of plasmablasts and early plasma cells (PCs), Compared to IL-2 or IL-10, IL-6 alone was inefficient at PC generation, However, when combined with IL-2 or IL-10, IL-6 enhanced generation of early PCs. Moreover, anti-IL-6 monoclonal antibody markedly reduced IL-2-induced generation of early plasma cells, but not of plasmablasts. These roles of IL-2 and IL-6 were consistent with the difference in the expression of their respective receptors (R), CD25 (IL-2R alpha) was increased 72 +/- 10-fold on activated B cells, but decreased and then disappeared on plasmablasts, Conversely, CD126 (IL-6R alpha) was barely expressed on activated B cells, but increased 18 +/- 2-fold on preplasmablasts, Finally, IL-6 enhanced the proliferation (2-fold increase) of IL-2-generated plasmablasts, In conclusion, the data indicate that IL-6 is a growth factor for nonmalignant human plasmablasts, (Blood, 2001; 97:1817-1822) (C) 2001 by The American Society of Hematology.