Time course of deterioration of rod and cone function in RCS rat and the effects of subretinal cell grafting: a light- and dark-adaptation study
Time course of deterioration of rod and cone function in RCS rat and the effects of subretinal cell grafting: a light- and dark-adaptation study
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DOI：
10.1016/j.visres.2004.08.023
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发表时间：
2005-02-01
期刊：
影响因子：
1.8
通讯作者：
Lund, RD
中科院分区：
文献类型：
--
作者：
Girman, S;Wang, S;Lund, RD
为了研究视杆细胞和视锥细胞功能在视网膜变性过程中以及视网膜下细胞移植后受到的不同影响，我们通过记录麻醉大鼠上级丘的阈值多单位反应获得了光适应和暗适应曲线。未手术的RCS营养不良和非营养不良大鼠和视网膜下移植的营养不良大鼠的细胞，已知限制感光细胞degeneration.The适应曲线表明，杆功能严重受损，在未手术的营养不良RCS大鼠在低亮度水平，甚至早在21天的年龄和视锥阈值逐渐升高，随着时间的推移。虽然细胞移植保留了视杆细胞和视锥细胞，但视杆细胞功能没有恢复，尽管阻止了视锥细胞阈值反应的进一步恶化。这引起了人们的关注，即外核层厚度的测量本身可能不是视觉能力和恢复策略有效性的准确测量。(C)2004爱思唯尔有限公司保留所有权利。
To examine how rod and cone function are differentially affected during retinal degeneration, and after subretinal cell grafting, we obtained light- and dark-adaptation curves by recording threshold multiunit responses from the superior colliculus of anesthetized rats. Unoperated RCS dystrophic and non-dystrophic rats were used and the effects of subretinal grafting in dystrophic rats of cells known to limit photoreceptor degeneration were examined.The adaptation curves showed that rod function was severely compromised in unoperated dystrophic RCS rats at low luminance levels, even as early as 21 days of age and that cone thresholds became gradually elevated over time. While cell transplantation preserved both rod and cone photoreceptors, rod function did not recover, although further deterioration of cone threshold responses was prevented. This raises concern that measures Of Outer nuclear layer thickness may not in themselves be an accurate measure of visual capabilities and efficacy of a restoration strategy. (C) 2004 Elsevier Ltd. All rights reserved.