FRET sensor-based quantification of intracellular trehalose in mammalian cells
FRET sensor-based quantification of intracellular trehalose in mammalian cells
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DOI：
10.1080/09168451.2015.1069699
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发表时间：
2016-01-02
影响因子：
1.6
通讯作者：
Kikawada, Takahiro
中科院分区：
文献类型：
--
作者：
Kikuta, Shingo;Hou, Bi-Huei;Kikawada, Takahiro
海藻糖在哺乳动物细胞中具有应激保护作用和自噬诱导作用。由于微摩尔水平的细胞内海藻糖难以定量，其作用的分子机制仍然不清楚。在这里，我们展示了一种新的基于FRET的海藻糖监测技术。在哺乳动物细胞中表达的FLIPsuc901Venus传感器能够快速、非破坏性地检测细胞内无极少量的海藻糖。
Trehalose acts as a stress protectant and an autophagy inducer in mammalian cells. The molecular mechanisms of action remain obscure because intracellular trehalose at micromolar level is difficult to quantitate. Here, we show a novel trehalose monitoring technology based on FRET. FLIPsuc901Venus sensor expressed in mammalian cells enables to quickly and non-destructively detect an infinitesimal amount of intracellular trehalose.