Differential distribution of endomorphin 1- and endomorphin 2-like immunoreactivities in the CNS of the rodent
Differential distribution of endomorphin 1- and endomorphin 2-like immunoreactivities in the CNS of the rodent
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DOI：
10.1002/(sici)1096-9861(19990322)405:4
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发表时间：
1999-03-22
影响因子：
2.5
通讯作者：
Zadina, JE
中科院分区：
文献类型：
--
作者：
Martin-Schild, S;Gerall, AA;Zadina, JE
内啡肽是一种内源性肽，对多阿片受体具有高亲和力和选择性，具有强镇痛活性。内啡肽1 （Tyr-Pro-Trp-Phe-NH2; EM1）和内啡肽2 （tyr - pro - phe - nh2; EM2）在大鼠中枢神经系统中的分布仅通过免疫细胞化学与两种抗血清测定，每种抗血清对目标抗原均表现出明显的偏好。表达em2样免疫反应性的核周存在于下丘脑后部，而表达em1样免疫反应性的核周存在于下丘脑后部和孤立束核（NTS）。em1样免疫反应性比em2样免疫反应性更广泛和密集地分布在整个大脑中，而em2样免疫反应性在脊髓中比em1样免疫反应性更普遍。臂旁核和NTS中em1样免疫反应纤维密度最大，在中隔、对角带、终纹床核、血管器、Meynert核、室旁丘脑核、下丘脑后核、导水管周围灰核、蓝斑核、伏隔核和杏仁核均有显著染色。em2样免疫反应纤维密度最大的部位为脊髓背角浅层和脊髓三叉束核。在大鼠、小鼠和豚鼠中，免疫反应的总体模式相似，但也存在一些差异。在许多但不是所有的部位，免疫反应纤维显著地存在于mu p受体集中的区域。神经解剖学结果表明，内啡肽参与调节伤害和自主神经系统的过程和应激反应。出版于1999年Wiley-Liss， Inc.dagger
Endomorphins are endogenous peptides that have high affinity and selectivity for the mu-opiate receptor and potent analgesic activity. The distributions of endomorphin 1 (Tyr-Pro-Trp-Phe-NH2; EM1) and endomorphin 2 (Tyr-Pro-Phe-Phe-NH2; EM2) in the rat central nervous system mere determined by immunocytochemistry with two antisera, each demonstrating clear preference for the target antigen. Perikarya expressing EM2-like immunoreactivity were present in the posterior hypothalamus, whereas those expressing EM1-like immunoreactivity were present in both the posterior hypothalamus and the nucleus of the solitary tract (NTS). EM1-like immunoreactivity was more widely and densely distributed throughout the brain than was EM2-like immunoreactivity, whereas EM2-like immunoreactivity was more prevalent in the spinal cord than was EM1-like immunoreactivity. The greatest density of EM1-like-immunoreactive fibers was detected in the parabrachial nucleus and the NTS, with notable staining in the septum, diagonal band, bed nucleus of the stria terminalis, organum vasculosum, nucleus of Meynert, paraventricular thalamic nucleus, posterior hypothalamic nucleus, periaqueductal gray, locus coeruleus, nucleus accumbens, and amygdala. The greatest density of EM2-like-immunoreactive fibers was detected in the superficial laminae of the spinal cord dorsal horn and the nucleus of the spinal trigeminal tract. The overall pattern of immunoreactivities was similar in rat, mouse, and guinea pig, but some differences were observed. In many but not in all locations, immunoreactive fibers were prominently present in regions in which mu p receptors are reported to be concentrated. The neuroanatomical results suggest that endomorphins participate in modulating nociceptive and autonomic nervous system processes and responsiveness to stress. Published 1999 Wiley-Liss, Inc.dagger