Chloride transport in mitochondrion-rich cells of euryhaline tilapia (Oreochromis mossambicus) larvae
Chloride transport in mitochondrion-rich cells of euryhaline tilapia (Oreochromis mossambicus) larvae
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DOI：
10.1152/ajpcell.00218.2009
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发表时间：
2009-10-01
影响因子：
5.5
通讯作者：
Lin, Li-Yih
中科院分区：
文献类型：
--
作者：
Horng, Jiun-Lin;Hwang, Pung-Pung;Lin, Li-Yih
洪 JL，黄 PP，施 TH，文 ZH，林 CS，林 LY。广盐罗非鱼（Oreochromis mossambicus）幼虫富含线粒体的细胞中的氯离子转运。 Am J Physiol Cell Physiol 297：C845-C854，2009。首次发表于 2009 年 8 月 5 日； doi:10.1152/ajpcell.00218.2009.-应用无创扫描离子选择电极技术 (SIET) 测量广盐罗非鱼 (Oreochromis mossambicus) 幼虫皮肤中单个富含线粒体的细胞 (MRC) 的 Cl- 转运。在适应海水 (SW) 的幼虫中，在表面测量了向外的 Cl- 梯度（20 类似于比背景高 80 mM），表明 Cl- 从皮肤分泌。通过对 MRC 和相邻角质形成细胞 (KC) 表面的连续探测，在 MRC 的顶端开口（膜）处检测到 Cl- 的显着向外通量。用100μM哇巴因或布美他尼治疗可抑制Cl-分泌约75%。在淡水 (FW) 适应的幼虫中，在皮肤表面测量到较低水平的向外 Cl- 梯度（0.2 类似于 1 mM）。低Cl-水（类似于0.005 mM）的驯化增加了幼虫皮肤中MRC的顶端Na+-Cl-协同转运蛋白（NCC）免疫反应性。当探测一组表达 NCC 的 MRC（凸 MRC）的外表面时，检测到 Cl- 的向内通量。 NCC 抑制剂（100 μM 美托拉宗）使通量降低了约 90%。这项研究为SW和FW驯化的广盐罗非鱼的MRC进行Cl-转运以及顶端NCC参与FW驯化的鱼的MRC吸收Cl-提供了直接和令人信服的证据。
Horng JL, Hwang PP, Shih TH, Wen ZH, Lin CS, Lin LY. Chloride transport in mitochondrion-rich cells of euryhaline tilapia (Oreochromis mossambicus) larvae. Am J Physiol Cell Physiol 297: C845-C854, 2009. First published August 5, 2009; doi:10.1152/ajpcell.00218.2009.-A noninvasive scanning ion-selective electrode technique (SIET) was applied to measure Cl- transport at individual mitochondrion-rich cells (MRCs) in the skin of euryhaline tilapia (Oreochromis mossambicus) larvae. In seawater (SW)-acclimated larvae, outward Cl- gradients (20 similar to 80 mM higher than the background) were measured at the surface, indicating a secretion of Cl- from the skin. By serial probing over the surface of MRCs and adjacent keratinocytes (KCs), a significant outward flux of Cl- was detected at the apical opening (membrane) of MRCs. Treatment with 100 mu M ouabain or bumetanide inhibited the Cl- secretion by similar to 75%. In freshwater (FW)-acclimated larvae, a lower level of outward Cl- gradients (0.2 similar to 1 mM) was measured at the skin surface. Low-Cl- water (similar to 0.005 mM) acclimation increased the apical Na+-Cl- cotransporter (NCC) immunoreactivity of MRCs in the larval skin. An inward flux of Cl- was detected when probing the exterior surface of a group of MRCs (convex-MRCs) that express the NCC. An NCC inhibitor (100 mu M metolazone) reduced the flux by similar to 90%. This study provides direct and convincing evidence for Cl- transport by MRCs of SW-and FW-acclimated euryhaline tilapia and the involvement of an apical NCC in Cl- uptake of MRCs of FW-acclimated fish.