Effects of antioxidants on the ischemia/reperfusion injury in rat
Effects of antioxidants on the ischemia/reperfusion injury in rat
复制标题
抗氧化剂对大鼠缺血/再灌注损伤的影响
DOI：
10.1007/s13318-010-0020-y
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
M.Hayashi
中科院分区：
文献类型：
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作者：
Y.Takizawa;H.Kishimoto;T.Kitazato;M.Tomita;M.Hayashi
小肠移植成功必须克服缺血再灌注（I/R）损伤。缺血再灌注（I/R）后产生的活性氧（ROS）可引起脂质过氧化反应，是导致黏膜损伤的原因之一。我们以前报道过抗氧化剂对I/R损伤的保护作用的体外研究。在本研究中，我们在体内研究中检查了抗氧化剂对肠I/R损伤的抑制作用。Wistar/ST大鼠采用弹簧秤和手术缝合法造成肠缺血1h，再灌注1h。我们使用4，5-二羟基-1，3-苯二磺酸（Tiron）、虾青素（ATX）和表没食子儿茶素没食子酸酯（EGCG）作为抗氧化剂。三种抗氧化剂对在体肠I/R引起的黏膜损伤、TJ开放及P-gp蛋白表达水平下降均有抑制作用。Tyron和EGCG抑制P-gp功能，而ATX不抑制P-gp功能。因此，对于在肠移植后使用P-gp底物如免疫抑制剂，不抑制P-gp的ATX被认为对肠I/R损伤有效。
Ischemia/reperfusion (I/R) injury must be overcome in order to succeed in small intestinal transplantation. Reactive oxygen species (ROS) are generated by I/R, and they induce lipid peroxidation which is one of the causes of mucosal lesion. We previously reported the protection effects of antioxidants to I/R injury in the in vitro study. In the present study, we examined the inhibitive effect of antioxidants on intestinal I/R injury in the in vivo study. Intestinal ischemia was induced in Wistar/ST rats using the spring scale and the surgical suture for 1 h, followed by reperfusion for 1 h. We used 4,5-dihydroxy-1,3-benzene-disulfonic acid (Tiron), astaxanthin (ATX) and epigallocatechin gallate (EGCG) as antioxidants. The inhibitive effects on mucosal lesion, opening of TJ and decrease in protein expression level of P-gp by in vivo intestinal I/R were admitted by three kinds of antioxidant. Tiron and EGCG inhibited P-gp function but ATX did not. Therefore, for the use of P-gp substrate like immunosuppressants after the intestinal transplantation, ATX, which does not inhibit P-gp is considered to be effective for intestinal I/R injury.