Intra- and extra-cellular organophosphorus hydrolase production with recombinant E-coli using fed-batch fermentation
Intra- and extra-cellular organophosphorus hydrolase production with recombinant E-coli using fed-batch fermentation
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DOI：
10.1007/s11274-008-9679-5
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发表时间：
2008-09-01
影响因子：
4.1
通讯作者：
Lee, Jung-heon
中科院分区：
文献类型：
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作者：
Jin, Li Hua;Kwun, Kyuhyuk;Lee, Jung-heon
研究了E. coli pET 812。在分批发酵的情况下，通过分批发酵获得的最大OPH浓度低至4 × 10（5）U/l，因为细胞生长和OPH产生被高初始浓度的葡萄糖抑制。为了开发用于获得更高浓度的OPH的补料分批发酵方法，间歇地或连续地添加高浓度的葡萄糖溶液（500 g/l）以增加碳源浓度。最终，在24小时内用补料分批发酵产生3.2 × 10（6）U/l的OPH。这比常规分批发酵的产率高8倍。OPH的总浓度为399 - 441 mg/l，比使用E.杆菌近一半（44%）的OPH分泌到培养液中。
A fed-batch fermentation process for the production of organophosphorus hydrolase (OPH) (EC 3.1.8.1) by E. coli pET812 was developed in this research. With batch fermentation, the maximum OPH concentrations attained by batch fermentation were as low as 4 x 10(5) U/l because cell growth and OPH production were inhibited by a high initial concentration of glucose. To develop a fed-batch fermentation process for obtaining higher concentrations of OPH, highly concentrated glucose solution (500 g/l) was added intermittently or continuously to increase the carbon source concentration. Eventually, 3.2 x 10(6) U/l of OPH was produced with fed-batch fermentation in 24 h. This was eight times higher than the yield with conventional batch fermentation. A total concentration of 399-441 mg of OPH was produced/l, which was four times higher than that reported when using E. coli. Nearly half (44%) of the produced OPH was secreted into the culture solution.