Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes
Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes
复制标题
DOI：
10.1016/j.cell.2005.12.038
复制
发表时间：
2006-03-10
期刊：
影响因子：
64.5
通讯作者：
Zhuang, XW
中科院分区：
文献类型：
--
作者：
Lakadamyali, M;Rust, MJ;Zhuang, XW
细胞的正常功能依赖于细胞内配体和受体的正确分选。早期的内生体一直被认为是用于降解的货物与用于回收的货物分开的初始分拣站。使用活细胞成像来实时监测单个内体和配体颗粒，我们发现了一种在内体进入早期之前发生的分选机制。我们发现早期的内小体实际上由两个不同的种群组成：一个是在微管上高度移动的动态种群，向晚内体快速成熟，以及一个静态种群，成熟得慢得多。一些要被降解的货物优先针对动态的内体，而循环配体转铁蛋白被非选择性地输送到所有早期的内体，并在更大的静态种群中有效地浓缩。这种早期的内小体分选过程始于包被网状蛋白的小泡，依赖于微管的运动，并且似乎涉及内吞适配器。
Cells rely on the correct sorting of endocytic ligands and receptors for proper function. Early endosomes have been considered as the initial sorting station where cargos for degradation separate from those for recycling. Using live-cell imaging to monitor individual endosomes and ligand particles in real time, we have discovered a sorting mechanism that takes place prior to early endosome entry. We show that early endosomes are in fact comprised of two distinct populations: a dynamic population that is highly mobile on microtubules and matures rapidly toward late endosomes and a static population that matures much more slowly. Several cargos destined for degradation are preferentially targeted to the dynamic endosomes, whereas the recycling ligand transferrin is nonselectively delivered to all early endosomes and effectively enriched in the larger, static population. This pre-early endosome sorting process begins at clathrin-coated vesicles, depends on microtubule-dependent motility, and appears to involve endocytic adaptors.