ISOLATION AND ULTRASTRUCTURE OF ALEURONE CELL-WALLS FROM WHEAT AND BARLEY
ISOLATION AND ULTRASTRUCTURE OF ALEURONE CELL-WALLS FROM WHEAT AND BARLEY
复制标题
DOI:
10.1071/pp9810453
复制
发表时间:
1981-01-01
期刊:
影响因子:
--
通讯作者:
STONE, BA
中科院分区:
文献类型:
--
作者:
BACIC, A;STONE, BA
Aleurone layers and cell walls from both wheat (T. aestivum L. cv. Insignia) and barley (H. vulgare L. cv. Clipper) were isolated by differential centrifugation in benzene CCl4 mixtures and by air classification. The isolated walls were obtained in sufficient quantities and purity for comprehensive chemical analysis. Morphological characteristics of the isolated aleurone layers and walls were examined by bright field, fluorescence and scanning electron microscopy and compared with their appearance in whole grains. Transmission electron microscopy of wall sections clearly showed their characteristic bilayered structure. Aniline blue-positive deposits were observed at the aleurone-starchy endosperm interfaces of both wheat and barley.