RIM101-dependent and -independent pathways govern pH responses in Candida albicans
RIM101-dependent and -independent pathways govern pH responses in Candida albicans
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DOI：
10.1128/mcb.20.3.971-978.2000
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发表时间：
2000-02-01
影响因子：
5.3
通讯作者：
Mitchell, AP
中科院分区：
文献类型：
--
作者：
Davis, D;Wilson, RB;Mitchell, AP
白色念珠菌在广泛的 pH 范围内生长和分化是其感染易感宿主的一系列组织的能力的基础。我们根据白色念珠菌 RIM101、RIM20 和 RIM8 与一种已知真菌 pH 响应途径成分的同源性，鉴定出它们。每个基因中的 PCR 产物破坏突变会导致对碱性 pH 值的三种反应出现缺陷：丝状形成、PRA1 和 PHR1 的诱导以及 PHR2 的抑制。我们发现RIM101本身是一个碱诱导基因，也依赖于Rim20p和Rim8p进行诱导。两项观察结果表明还存在一种新的 pH 响应途径。首先，在 Rim101p、Rim20p 或 Rim8p 缺失的情况下，PHR2 成为碱诱导基因。其次，我们创建了 Rim101p 活性独立于 Rim20p 和 Rim8p 的菌株；在这些菌株中，丝状形成仍然依赖于 pH 值。因此，pH 通过 RIM101 依赖性和 RIM101 独立途径控制白色念珠菌的基因表达和细胞分化。
Growth and differentiation of Candida albicans over a broad pH range underlie its ability to infect an array of tissues in susceptible hosts. We identified C. albicans RIM101, RIM20, and RIM8 based on their homology to components of the one known fungal pH response pathway. PCR product-disruption mutations in each gene cause defects in three responses to alkaline pH: filamentation, induction of PRA1 and PHR1, and repression of PHR2. We find that RIM101 itself is an alkaline-induced gene that also depends on Rim20p and Rim8p for induction. Two observations indicate that a novel pH response pathway also exists. First, PHR2 becomes an alkaline-induced gene in the absence of Rim101p, Rim20p, or Rim8p. Second, we created strains in which Rim101p activity is independent of Rim20p and Rim8p; in these strains, filamentation remains pH dependent. Thus, pH governs gene expression and cellular differentiation in C. albicans through both RIM101-dependent and RIM101-independent pathways.