The receptor preference of influenza viruses.
The receptor preference of influenza viruses.
复制标题
DOI：
10.1111/j.1750-2659.2010.00130.x
复制
发表时间：
2010-05-01
影响因子：
4.4
通讯作者：
Dimmock NJ
中科院分区：
文献类型：
--
作者：
Meng B;Marriott AC;Dimmock NJ
请引用这篇论文：孟等。（2010）流感病毒的受体偏好。流感和其他呼吸道病毒4（3），147-153。 目的流感病毒用来感染细胞的细胞表面受体是一个N-乙酰神经氨酸（NANA）残基，其末端通过α 2，3或α 2，6键与糖蛋白或糖脂的碳水化合物部分相连。 我们的目的是确定一种快速和技术上简单的方法来确定整个甲型流感病毒颗粒的细胞受体利用率。 方法我们采用表面等离子体共振检测病毒与胎球蛋白的结合，胎球蛋白是一种天然存在的糖蛋白，具有α 2，3-和α 2，6-连接的NANA，以及游离的3′-唾液酸乳糖或6′-唾液酸乳糖来竞争病毒结合。 所有病毒原种均在鸡胚中产生。 结果流感病毒与NANA α 2，3Gal或NANA α 2，6 Gal优先结合，或无优先结合。 两种PR 8病毒具有不同的结合偏好。病毒的结合偏好与其已知的生物学特性密切相关。 结论我们的数据表明，预测流感病毒对受体的利用并不容易。 然而，本文所述的直接实验测定可以为与病毒发病机制、生物学和结构有关的实验提供信息。原则上，该方法可用于与末端NANA残基结合的任何病毒。
Please cite this paper as: Meng et al. (2010) The receptor preference of influenza viruses. Influenza and Other Respiratory Viruses 4(3), 147–153. Objectives The cell surface receptor used by an influenza virus to infect that cell is an N‐acetyl neuraminic acid (NANA) residue terminally linked by an alpha2,3 or alpha2,6 bond to a carbohydrate moiety of a glycoprotein or glycolipid. Our aim was to determine a quick and technically simple method to determine cell receptor usage by whole influenza A virus particles. Methods We employed surface plasmon resonance to detect the binding of viruses to fetuin, a naturally occurring glycoprotein that has both alpha2,3‐ and alpha2,6‐linked NANA, and free 3′‐sialyllactose or 6′‐sialyllactose to compete virus binding. All virus stocks were produced in embryonated chicken’s eggs. Results The influenza viruses tested bound preferentially to NANAalpha2,3Gal or to NANAalpha2,6Gal, or showed no preference. Two PR8 viruses had different binding preferences. Binding preferences of viruses correlated well with their known biological properties. Conclusions Our data suggest that it is not easy to predict receptor usage by influenza viruses. However, direct experimental determination as described here can inform experiments concerned with viral pathogenesis, biology and structure. In principle, the methodology can be used for any virus that binds to a terminal NANA residue.