Circulating extracellular vesicles in the aging process: impact of aerobic exercise
Circulating extracellular vesicles in the aging process: impact of aerobic exercise
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DOI：
10.1007/s11010-017-3160-4
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发表时间：
2018-03-01
影响因子：
4.3
通讯作者：
Siqueira, Ionara Rodrigues
中科院分区：
文献类型：
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作者：
Bertoldi, Karine;Cechinel, Laura Reck;Siqueira, Ionara Rodrigues
本研究旨在探讨短暂和延迟运动对衰老过程中血清细胞外囊泡（EVs）的影响。将3月龄、21月龄和26月龄的雄性Wistar大鼠分为运动组和安静组。运动方案包括每日中度跑步机运动（2周内每天20分钟）。在最后一次运动后1和18 h收集躯干血，并获得循环EV。CD63水平和乙酰胆碱酯酶（AChE）活性被用作外泌体（EV的一种亚型）的标志物。此外，还评价了淀粉样蛋白β（A β）水平和氧化状态参数、特异性反应物质含量、超氧化物歧化酶（SOD）活性和SOD 1含量的定量。与年轻大鼠相比，老年大鼠在循环外泌体中表现出降低的CD 63水平和增加的AChE活性。此外，在老年大鼠的循环EV中发现了更高的反应性物质水平。延迟运动的影响，观察外周EV，因为CD63，活性物质的含量，和乙酰胆碱酯酶活性改变后18小时，最后一次运动。我们的研究结果表明，健康的老龄化过程可以改变循环EV的配置文件，运动诱导的有益效果可能与其调制EV。
Our aim was to investigate transitory and delayed exercise effects on serum extracellular vesicles (EVs) in aging process. Male Wistar rats of 3-, 21-, and 26-month old were allocated into exercised and sedentary groups. The exercise protocol consisted in a daily moderate treadmill exercise (20 min daily during 2 weeks). Trunk blood was collected 1 and 18 h after the last exercise session, and circulating EVs were obtained. CD63 levels and acetylcholinesterase (AChE) activity were used as markers of exosome, a subtype of EVs. In addition, the quantification of amyloid-beta (A beta) levels and the oxidative status parameters, specifically reactive species content, superoxide dismutase (SOD) activity, and SOD1 content were evaluated. Aged rats showed reduced CD63 levels and increased AChE activity in circulating exosomes compared to young ones. Moreover, higher reactive species levels were found in circulating EVs of aged rats. Delayed exercise effects were observed on peripheral EVs, since CD63, reactive species content, and AChE activity were altered 18 h after the last exercise session. Our results suggest that the healthy aging process can modify circulating EVs profile, and exercise-induced beneficial effects may be related to its modulation on EVs.