Global transcriptome analysis of H5N1 influenza virus-infected human cells
Global transcriptome analysis of H5N1 influenza virus-infected human cells
复制标题
H5N1流感病毒感染的人类细胞的全局转录组分析
DOI：
10.1186/s41065-019-0085-9
复制
发表时间：
2019-02-06
期刊：
影响因子：
2.7
通讯作者：
Li, Jing
中科院分区：
文献类型：
--
作者：
Cao, Ying;Zhang, Kun;Li, Jing
背景甲型流感病毒(IAV)主要属于正粘病毒科。IAV在人类中引起一种高度传染性的呼吸道疾病，在全球造成严重的经济损失。结果A/斑头雁/青海/1/2005(A/QH)株能感染A549和293 T细胞，其中A549细胞感染率较高。为了确定人类细胞对流感感染的宿主细胞反应，利用RNA测序技术鉴定了禽流感病毒感染组和未感染对照组之间的差异表达基因(Deg)。基因本体论和京都百科全书的基因和基因组途径分析显示，DEGS主要与细胞功能和代谢过程有关，而可能与宿主细胞反应有关的细胞功能包括对病毒的防御反应和蛋白质修饰。结论整体转录组分析结果揭示了细胞感染流感病毒的敏感基因和途径，为进一步研究IAV与宿主细胞的复杂关系提供了进一步的证据。
BackgroundInfluenza A virus (IAV) belongs to theOrthomyxoviridaefamily. IAV causes a highly contagious respiratory disease in humans that exacts severe economic losses globally. The virus uses strategies developed to exploit and subvert cellular proteins and pathways to increase its own replication and to inhibit antiviral immune response.ResultsA/bar-headed goose/Qinghai/1/2005 (A/QH) was able to infect A549 and 293 T cells, with a high infection rate for A549 cells. To identify host cellular responses of human cells to influenza infection, differentially expressed genes (DEGs) between AIV-infected groups and uninfected controls were identified using RNA-sequencing. The DEGs were annotated by Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway analyses, which revealed that the DEGs were mainly linked to cellular function and metabolic processes, while the cellular function that is probably associated with host cellular response of human cells, including defense response to virus and protein modification. All the DEGs and pathways were possibly involved in the response to IAV invasion.ConclusionsThe global transcriptome analysis results revealed that sensitive genes and pathways of the cells were infected with the influenza virus and provided further evidence to investigate the complicated relationship between IAV and host cells.