MAIRA- real-time taxonomic and functional analysis of long reads on a laptop
MAIRA- real-time taxonomic and functional analysis of long reads on a laptop
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DOI：
10.1186/s12859-020-03684-2
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发表时间：
2020-09-17
影响因子：
3
通讯作者：
Huson, Daniel H.
中科院分区：
文献类型：
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作者：
Albrecht, Benjamin;Bagci, Caner;Huson, Daniel H.
背景移动测序设备和笔记本电脑性能的进步使该领域的宏基因组测序和分析成为技术上可行的前景。然而，宏基因组分析管道通常设计为在服务器和云中运行。结果MAIRA 是一个新的独立程序，用于在笔记本电脑上对长读宏基因组测序数据进行交互式分类和功能分析，无需外部资源。该程序执行快速、在线、属级分析，以及按需、详细的分类和功能分析。它使用两个级别的 DNA 读数与蛋白质参考序列的移码感知比对，然后使用蛋白质同线性图进行详细分析。结论我们预计，当难以访问服务器或云设施时，该软件将由该领域的研究人员使用，或者由不经常访问此类设施的个人使用，例如医学研究人员、作物科学家或教师。
BackgroundAdvances in mobile sequencing devices and laptop performance make metagenomic sequencing and analysis in the field a technologically feasible prospect. However, metagenomic analysis pipelines are usually designed to run on servers and in the cloud.ResultsMAIRA is a new standalone program for interactive taxonomic and functional analysis of long read metagenomic sequencing data on a laptop, without requiring external resources. The program performs fast, online, genus-level analysis, and on-demand, detailed taxonomic and functional analysis. It uses two levels of frame-shift-aware alignment of DNA reads against protein reference sequences, and then performs detailed analysis using a protein synteny graph.ConclusionsWe envision this software being used by researchers in the field, when access to servers or cloud facilities is difficult, or by individuals that do not routinely access such facilities, such as medical researchers, crop scientists, or teachers.