Molecular, biological, and antigenic characterization of a Border disease virus isolated from a pig during classical swine fever surveillance in Japan
Molecular, biological, and antigenic characterization of a Border disease virus isolated from a pig during classical swine fever surveillance in Japan
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DOI：
10.1177/1040638714541837
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发表时间：
2014-07-01
影响因子：
1.5
通讯作者：
Shirai, Junsuke
中科院分区：
文献类型：
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作者：
Nagai, Makoto;Aoki, Hiroshi;Shirai, Junsuke
在本研究中，进行了分子、生物学和抗原分析，以表征2012年在日本从猪中分离的边境病病毒（BDV）FNK 2012 -1株。完整的基因组包含12，327个核苷酸（nt），包括11，685 nt的大开放阅读框。系统进化树分析表明，FNK 2012 -1与绵羊BDV基因型1聚为一类。FNK 2012 -1在猪、牛和绵羊原代细胞和细胞系中生长，但在牛和绵羊细胞中的生长优于猪细胞。接种FNK 2012 -1的无特定病原体猪未显示任何临床体征。未接种的接触对照猪也未显示临床体征，且未发生血清转化。结果表明，FNK 2012 -1可能是反刍动物来源的，对猪的适应性较差。这些观察结果可以为BDV在猪中的感染和传播提供重要的证据，BDV可能是反刍动物来源的。
In the current study, molecular, biological, and antigenic analyses were performed to characterize Border disease virus (BDV) strain FNK2012-1 isolated from a pig in 2012 in Japan. The complete genome comprises 12,327 nucleotides (nt), including a large open reading frame of 11,685 nt. Phylogenetic analysis revealed that FNK2012-1 was clustered into BDV genotype 1 with ovine strains. FNK2012-1 grew in porcine, bovine, and ovine primary cells and cell lines, but grew better in bovine and ovine cells than in porcine cells. Specific pathogen-free pigs inoculated with FNK2012-1 did not show any clinical signs. Noninoculated contact control pigs also did not show clinical signs and did not seroconvert. The results suggest that FNK2012-1 may be of ruminant origin and is poorly adapted to pigs. Such observations can provide important insights into evidence for infection and transmission of BDV, which may be of ruminant origin, among pigs.