Heterologous RNA replication enhancer stimulates in vitro RNA synthesis and template-switching by the carmovirus, but not by the tombusvirus, RNA-dependent RNA polymerase: Implication for modular evolution of RNA viruses
Heterologous RNA replication enhancer stimulates in vitro RNA synthesis and template-switching by the carmovirus, but not by the tombusvirus, RNA-dependent RNA polymerase: Implication for modular evolution of RNA viruses
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DOI：
10.1016/j.virol.2005.06.042
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发表时间：
2005-10-10
期刊：
影响因子：
3.7
通讯作者：
Nagy, PD
中科院分区：
文献类型：
--
作者：
Cheng, CP;Panavas, T;Nagy, PD
病毒RNA在RNA病毒复制过程中发挥着多重作用，作为互补RNA合成的模板，促进病毒复制酶复合体的组装。这些作用由顺式作用的调控元件协调，如启动子和复制增强子（REN）。为了检验这些RNA元件是否可以被相关的病毒RNA依赖性RNA聚合酶（RdRp）利用，我们在体外比较了同源和异源REN元件对tombus-（黄瓜坏死病毒，CNV）、carmovirus（萝卜皱病毒，TCV）和丙型肝炎病毒（HCV） RdRps的互补RNA合成和模板转换的潜在刺激作用。CNV RdRp选择性地利用其同源的REN，而对异源的TCV REN进行区分。相反，TCV REN和外源tombusvirus REN刺激TCV RdRp的RNA合成效率相当。异种REN元素也促进了TCV和HCV RdRps的体外模板转换。基于这些观察结果，我们提出REN元件可以促进病毒间重组和新重组病毒的重组后扩增。(c) 2005爱思唯尔公司版权所有。
The viral RNA plays multiple roles during replication of RNA viruses, serving as a template for complementary RNA synthesis and facilitating the assembly of the viral replicase complex. These roles are coordinated by cis-acting regulatory elements, such as promoters and replication enhancers (REN). To test if these RNA elements can be used by related viral RNA-dependent RNA polymerases (RdRp), we compared the potential stimulatory effects of homologous and heterologous REN elements on complementary RNA synthesis and template-switching by the tombus- (Cucumber necrosis virus, CNV), carmovirus (Turnip crinkle virus, TCV) and hepatitis C virus (HCV) RdRps in vitro. The CNV RdRp selectively utilized its cognate REN, while discriminating against the heterologous TCV REN. On the contrary, RNA synthesis by the TCV RdRp was stimulated by the TCV REN and the heterologous tombusvirus REN with comparable efficiency. The heterologous REN elements also promoted in vitro template-switching by the TCV and HCV RdRps. Based on these observations, we propose that REN elements could facilitate intervirus recombination and postrecombinational amplification of new recombinant viruses. (c) 2005 Elsevier Inc. All rights reserved.