Curcumin Exerts Antidifferentiation Effect through AMPKα-PPAR-γ in 3T3-L1 Adipocytes and Antiproliferatory Effect through AMPKα-COX-2 in Cancer Cells
Curcumin Exerts Antidifferentiation Effect through AMPKα-PPAR-γ in 3T3-L1 Adipocytes and Antiproliferatory Effect through AMPKα-COX-2 in Cancer Cells
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DOI：
10.1021/jf802737z
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发表时间：
2009-01-14
影响因子：
6.1
通讯作者：
Park, Ock J.
中科院分区：
文献类型：
--
作者：
Lee, Yun K.;Lee, Won S.;Park, Ock J.
据报道，姜黄素具有预防肥胖和癌症的潜力。研究了AMP活化蛋白激酶(AMPK)调节的抑制姜黄素脂肪细胞分化或癌细胞增殖的下游靶点。姜黄素对AMPK的激活对于抑制脂肪细胞和癌细胞的分化或生长是至关重要的。姜黄素刺激AMPK后，3T3-L1脂肪细胞PPAR-γ表达下调，MCF-7细胞COX-2表达减少。人工合成的AMPK激活剂的应用也支持了AMPK在3T3-L1脂肪细胞中作为PPAR-γ上游信号的证据。在癌细胞中，AMPK被发现作为ERK1/2、p38和COX-2的调节器。姜黄素对AMPK及其下游靶标如PPAR-γ、MAPKASES和COX-2的调节在控制脂肪细胞和癌细胞方面似乎是重要的。
Curcumin has been reported to have the potential to prevent obesity as well as cancers. The downstream targets regulated by AMP-activated protein kinase (AMPK) for inhibiting adipocyte differentiation or cancer cell proliferation of curcumin were investigated. The activation of AMPK by curcumin was crucial for the inhibition of differentiation or growth in both adipocytes and cancer cells. Stimulation of AMPK by curcumin resulted in the down-regulation of PPAR (peroxisome proliferator-activated receptor)-gamma in 3T3-L1 adipocytes and the decrease in COX-2 in MCF-7 cells. Application of a synthetic AMPK activator also supported the evidence that AMPK acts as an upstream signal of PPAR-gamma in 3T3-L1 adipocytes. In cancer cells, AMPK was found to act as a regulator of ERK1/2, p38, and COX-2. Regulation of AMPK and its downstream targets such as PPAR-gamma, Mapkinases, and COX-2 by curcumin appears to be important in controlling adipocytes and cancerous cells.